Modomics - A Database of RNA Modifications

ID Card:

Full name: Ribosomal RNA small subunit methyltransferase G
Synonym: gidB
GI: 121190
COG: COG0357
UniProt: P25813
Structures: | 1XDZ |
Alpha Fold Predicted Structure: AF-P25813-F1
Enzyme type: methyltransferase
Position of modification - modification: s:533(527) - m7G


PDB Structures:


1XDZ

Structure Description:

Title: Crystal Structure of Gram_Positive Bacillus subtilis Glucose inhibited Division protein B (gidB), Structural genomics, MCSG
Classification: TRANSFERASE
Technique: X-Ray Diffraction
Resolution: 1.6
R value free: 0.221
R value observed: 0.203
R value work: 0.202

Abstract of the PDB Structure's related Publication:



Download RCSB-PDB Structures:

Pdb Files   1XDZ.pdb  
Pdbx/mmCIF Files   1XDZ.cif  


Protein sequence:

MNIEEFTSGLAEKGISLSPRQLEQFELYYDMLVEWNEKINLTSITEKKEVYLKHFYDSITAAFYVDFNQVNTICDVGAGAGFPSLPIKICFPHLHVTIVDSLNKRITFLEKLSEALQLENTTFCHDRAETFGQRKDVRESYDIVTARAVARLSVLSELCLPLVKKNGLFVALKAASAEEELNAGKKAITTLGGELENIHSFKLPIEESDRNIMVIRKIKNTPKKYPRKPGTPNKSPIEG

Comments:

RsmG is responsible for the synthesis of m7G535 (position 527 in E. coli) in the universally conserved loop of hairpin 18 in domain I. This modified nucleotide plays an essential role in binding antibiotics like streptomycin and kasugamycin. However the dependence on RsmG for antibiotic binding depends on the activity of RsmA (KsgA) catalyzing the formation of m6,6A1518,1519 at the 3'-end of 16S rRNA by RsmA (KsgA). In B. subtilis, mutations in both RsmA and RsmG allow better cell viability in the presence of antibiotics. AdoMet is the methyl group donor.





Alpha Fold Predicted Structure:




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Protein sequence:

M N I E E F T S G L A E K G I S L S P R Q L E Q F E L Y Y D M L V E W N E K I N L T S I T E K K E V Y L K H F Y D S I T A A F Y V D F N Q V N T I C D V G A G A G F P S L P I K I C F P H L H V T I V D S L N K R I T F L E K L S E A L Q L E N T T F C H D R A E T F G Q R K D V R E S Y D I V T A R A V A R L S V L S E L C L P L V K K N G L F V A L K A A S A E E E L N A G K K A I T T L G G E L E N I H S F K L P I E E S D R N I M V I R K I K N T P K K Y P R K P G T P N K S P I E G
20406080100120140160180200220SequenceGHITSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P25813-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P25813-F1.cif  
DSSP Secondary Structures   P25813.dssp  





Publications: