Modomics - A Database of RNA Modifications

Full name:	tRNA (guanine(26)-N(2))-dimethyltransferase
Synonym:	PH1829
GI:	14591578
Orf:
COG:	COG1867
UniProt:	O59493
Structures:	\| 2DUL \| 2EJT \| 2EJU \| 2YTZ \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	t:26 - m2,2G

Comments:

The activity was tested on in vitro transcribed tRNAVal.

Protein sequence:

MVNLELIEVQEGKAKILIPKAESIYDSPVFYNPRMALNRDIVVVLLNILNPKIVLDALSATGIRGIRFALETPAEEVWLNDISEDAYELMKRNVMLNFDG
ELRESKGRAILKGEKTIVINHDDANRLMAERHRYFHFIDLDPFGSPMEFLDTALRSAKRRGILGVTATDGAPLCGAHPRACLRKYLAVPLRGELCHEVGT
RILVGVIARYAAKYDLGIDVILAYYKDHYFRAFVKLKDGARKGDETLEKLGYIYFDDKTGKFELEQGFLPTRPNAYGPVWLGPLKDEKIVSKMVKEAESL
SLARKKQALKLLKMIDQELDIPLFYDTHAIGRRLKIETKKVEEIISALREQGYEATRTHFSPTGIKTSAPYEVFIETIKRI

Enzymatic activities:

Reaction	Substrate	Type	Position
G:m2G	tRNA (t)	Val/unknown/prokaryotic cytosol	26
m2G:m2,2G	tRNA (t)	Val/unknown/prokaryotic cytosol	26

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Crystal structure of tRNA N2,N2-guanosine dimethyltransferase Trm1 from Pyrococcus horikoshii.	Ihsanawati, Nishimoto M, Higashijima K, Shirouzu M, Grosjean H, Bessho Y, Yokoyama S	J Mol Biol	[details]	18789948	-