Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA (guanine(10)-N2)-dimethyltransferase
Synonym: (Pab)Trm-G10, PYRAB16240
GI: 327488511
Orf: PAB1283
COG: COG1041
UniProt: Q9UY84
Alpha Fold Predicted Structure: AF-Q9UY84-F1
Enzyme type: methyltransferase
Position of modification - modification: t:10 - m2,2G



Protein sequence:

MFYVEILGLLPEMAEAEVKALAELRNGTIKERDYLLVVGNVENVEIFERLGLAHEYGILLGSGDDVRDILDLVRGLEWKEIIKGTFAVRKEVMVNCAHEVKNLEKIIGGIIHSQGLRVNLSKPDTIIKVYCGRKLWIGIRIREFRGKEFDERKADRRPFSRPIALPPRIARAMVNLTRATREILDPFMGTGGMLIEAGLMGLKVYGIDIREDMVEGAKINLEYYGVKDYVVKVGDATKIKEAFPGKTFEAIATDPPYGTSTTLPMDRDELYKRALESMYSVLEGRLAIAFPSDFDALDVAETIGFKVIGRFYQRVHSSLSRYFYIMEAR

Comments:

TrmG10 it is the homolog of yeast Trm11. It catalyses formation of m2G10 or m2,2G10, depending on tRNA substrate. The activity was tested on heterologous substrates.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
G:m2G tRNA (t) 10 15210688   
m2G:m2,2G tRNA (t) 10 15210688   

Alpha Fold Predicted Structure:






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Protein sequence:

M F Y V E I L G L L P E M A E A E V K A L A E L R N G T I K E R D Y L L V V G N V E N V E I F E R L G L A H E Y G I L L G S G D D V R D I L D L V R G L E W K E I I K G T F A V R K E V M V N C A H E V K N L E K I I G G I I H S Q G L R V N L S K P D T I I K V Y C G R K L W I G I R I R E F R G K E F D E R K A D R R P F S R P I A L P P R I A R A M V N L T R A T R E I L D P F M G T G G M L I E A G L M G L K V Y G I D I R E D M V E G A K I N L E Y Y G V K D Y V V K V G D A T K I K E A F P G K T F E A I A T D P P Y G T S T T L P M D R D E L Y K R A L E S M Y S V L E G R L A I A F P S D F D A L D V A E T I G F K V I G R F Y Q R V H S S L S R Y F Y I M E A R

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q9UY84-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q9UY84-F1.cif  
DSSP Secondary Structures   Q9UY84.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
THUMP from archaeal tRNA:m22G10 methyltransferase, a genuine autonomously folding domain. Gabant G, Auxilien S, Tuszynska I, Locard M, Gajda MJ, Chaussinand G, Fernandez B, Dedieu A, Grosjean H, Golinelli-Pimpaneau B, Bujnicki JM, Armengaud J Nucleic Acids Res [details] 16687654 -
N2-methylation of guanosine at position 10 in tRNA is catalyzed by a THUMP domain-containing, S-adenosylmethionine-dependent methyltransferase, conserved in Archaea and Eukaryota. Armengaud J, Urbonavicius J, Fernandez B, Chaussinand G, Bujnicki JM, Grosjean H J Biol Chem [details] 15210688 -