Modomics - A Database of RNA Modifications

Full name:	tRNA (guanine(6)-N2)-methyltransferase
Synonym:	Trm14, MJ0438
GI:	15668614
Orf:
COG:	COG0116
UniProt:	Q57880
Structures:	\| \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	t:6 - m2G

Comments:

Distant homologs of Trm14 were also identified in eykaryota known to possess the m2G6 tRNA modification

Protein sequence:

MDYYVTLSPGLEKISKNEIESFGGKIKEIRENKGRIFFSGDLKLIPKINYLSRTIERMNILLHREEIPNIALDDIYKRVYNIDWTEWIKENQSFAIRPLR
AGEHNFTSIDIGRVAGEAVIKSYQRDKNIRLKVNLDEPDVIVRVEVIFDELIVGIDTTGDIALDKRGYRVFNHPAHLNATIASSLVYLSDWKDDEMLLDP
MCGSGTIPIEGALMKRNIPPGKFRENKYGFKFIDIFGYELLDKIKKEIVENKNIYKIIGLDKNQKYLDGAKDNAKNAEVLDTIEFICGDATKLHEKFNES
DVIIANPPYGIRIGSKRSVKKLYDEFLSSAKEIMHGSSRLIVITAEDKMFKDAIAKNNFEVKEEFNVMFGGLMTRVFYLTL

Enzymatic activities:

Reaction	Substrate	Type	Position
G:m2G	tRNA (t)	Cys/unknown/prokaryotic cytosol	6

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Formation of m2G6 in Methanocaldococcus jannaschii tRNA catalyzed by the novel methyltransferase Trm14.	Menezes S, Gaston KW, Krivos KL, Apolinario EE, Reich NO, Sowers KR, Limbach PA, Perona JJ	Nucleic Acids Res	[details]	21693558	-