| Full name: |
tRNA N6-threonylcarbamoyladenosine(37) synthesis protein |
| Synonym: |
YgjD |
| GI: |
16130960 |
| Orf: |
|
| COG: |
COG0533 |
| UniProt: |
P05852 |
| Structures: |
|
|
|
| Complex: |
TsaBDE |
| Enzyme type: |
threonylcarbamoyltransferase |
| Position of modification - modification: |
t:37 - t6A
|
Comments:
TsaD, the ortholog of eukaryal/archaeal Kae1, is a weak ATPase (produces ADP) and binds A37-containing tRNA. TsaD forms an essential interaction network with TsaB (YeaZ, a paralog of TsaD) and TsaE (YjeE) proteins. Together with TsaC (multiprotein complex), ATP, threonine and bicarbonate as cofactors, they catalyze the in vitro formation of t6A (threonyl-carbamoylation) at position 37 of bacterial tRNA decoding ANN codons (ile, Met, Thr, Lys, Asn, Ser and Arg).
Protein sequence:
MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVPELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAF
AWDVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDDAAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRP
MTDRPGLDFSFSGLKTFAANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLRAKLAEMMKKRRGEVFYARPEFCTD
NGAMIAYAGMVRFKAGATADLGVSVRPRWPLAELPAA
Enzymatic activities:
| Reaction
| Substrate
| Type
| Position
|
|
A:t6A
|
tRNA (t) |
|
37 |
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| Biosynthesis of threonylcarbamoyl adenosine (t6A), a universal tRNA nucleoside. |
Deutsch C, El Yacoubi B, de Crecy-Lagard V, Iwata-Reuyl D |
J Biol Chem |
[details]
|
22378793
|
-
|
| A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification. |
El Yacoubi B, Hatin I, Deutsch C, Kahveci T, Rousset JP, Iwata-Reuyl D, Murzin AG, de Crecy-Lagard V |
EMBO J |
[details]
|
21285948
|
-
|
| Effects on transcription of mutations in ygjD, yeaZ, and yjeE genes, which are involved in a universal tRNA modification in Escherichia coli. |
Hashimoto C, Sakaguchi K, Taniguchi Y, Honda H, Oshima T, Ogasawara N, Kato J |
J Bacteriol |
[details]
|
21873492
|
-
|
| Mechanism of N6-threonylcarbamoyladenonsine (t(6)A) biosynthesis: isolation and characterization of the intermediate threonylcarbamoyl-AMP. |
Lauhon CT... |
Biochemistry |
[details]
|
23072323
|
-
|
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