This ortholog of E. coli TsaE (YjeE) belongs to P-loop ATPases. It binds ATP and displays a weak ATPase activity. Purified recombinant TsaE oligomerizes in vitro. In bacteria four proteins: TsaD, Sua5, TsaE and TsaB (YgjD, YrdC, YjeE, and YeaZ, respectively) are both necessary and sufficient for t6A biosynthesis in vitro. YrdC and YgjD are members of universally conserved families while YeaZ and YjeE are specific to bacteria.