Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA pseudouridine synthase Pus10
Synonym: PusX
GI: 74536148
COG: COG1258
UniProt: Q8U1R6
Alpha Fold Predicted Structure: AF-Q8U1R6-F1
Enzyme type: pseudouridine synthase
Position of modification - modification: t:54 - Y
t:55 - Y



Protein sequence:

MILEKAREILEEHQLCNHCLGRLFGKLGKGTNEERGRAIRLLLSMETGKEYKEPEKCELCGGVFNNLDKFAELCIKAAEGIEFETFWVGSRFPEEIEKKEEEIWRKFRVVSGEKITKEFNRELGKVIAVRYGKTPVKERPDVVFIVEPFSEKVELQVNPIYVAGRYRKLIRGIPQTPAPGFKESIATIICRAFKKHFHGKCIFKGAGREDVDVRMLGNGRPFVVEIKRPRKRKVNLKDIEEEINQSGKVEVLNLRFITPEEAERILTTRHRKVYEAIVYVKDGITKEEVEKVVKSLKNAEIKQRTPRRVLNSRADLVRVRKVYDVKGELIDDKHFKLRLVTDGGLYIKELISGDRGRTTPSVSEILGKEAWCEILDVLEVLDDVEGDN

Comments:

Initially discovered as an enzyme specific for the formation of pseudouridine at position 55 in the T-loop of archaeal tRNA (Roovers et al., 2006), it was later shown to also catalyze the formation of pseudouridine at position 54 (Gurha & Gupta 2008). Pus10 is universally found in Archaea and only in few Eukarya, however in this last case the real function of eukaryal Pus10 in still unknown.





Alpha Fold Predicted Structure:




Downloading... [212730/363398]


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Protein sequence:

M I L E K A R E I L E E H Q L C N H C L G R L F G K L G K G T N E E R G R A I R L L L S M E T G K E Y K E P E K C E L C G G V F N N L D K F A E L C I K A A E G I E F E T F W V G S R F P E E I E K K E E E I W R K F R V V S G E K I T K E F N R E L G K V I A V R Y G K T P V K E R P D V V F I V E P F S E K V E L Q V N P I Y V A G R Y R K L I R G I P Q T P A P G F K E S I A T I I C R A F K K H F H G K C I F K G A G R E D V D V R M L G N G R P F V V E I K R P R K R K V N L K D I E E E I N Q S G K V E V L N L R F I T P E E A E R I L T T R H R K V Y E A I V Y V K D G I T K E E V E K V V K S L K N A E I K Q R T P R R V L N S R A D L V R V R K V Y D V K G E L I D D K H F K L R L V T D G G L Y I K E L I S G D R G R T T P S V S E I L G K E A W C E I L D V L E V L D D V E G D N
50100150200250300350SequenceGHTSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q8U1R6-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q8U1R6-F1.cif  
DSSP Secondary Structures   Q8U1R6.dssp  





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