ID Card:
Full name:
tRNA pseudouridine synthase Pus10
Synonym:
PusX
GI:
74536148
COG:
COG1258
UniProt:
Q8U1R6
Alpha Fold Predicted Structure:
AF-Q8U1R6-F1
Enzyme type:
pseudouridine synthase
Position of modification - modification:
t :54 - Y t :55 - Y
Protein sequence:
MILEKAREILEEHQLCNHCLGRLFGKLGKGTNEERGRAIRLLLSMETGKEYKEPEKCELCGGVFNNLDKFAELCIKAAEGIEFETFWVGSRFPEEIEKKEEEIWRKFRVVSGEKITKEFNRELGKVIAVRYGKTPVKERPDVVFIVEPFSEKVELQVNPIYVAGRYRKLIRGIPQTPAPGFKESIATIICRAFKKHFHGKCIFKGAGREDVDVRMLGNGRPFVVEIKRPRKRKVNLKDIEEEINQSGKVEVLNLRFITPEEAERILTTRHRKVYEAIVYVKDGITKEEVEKVVKSLKNAEIKQRTPRRVLNSRADLVRVRKVYDVKGELIDDKHFKLRLVTDGGLYIKELISGDRGRTTPSVSEILGKEAWCEILDVLEVLDDVEGDN
Comments:
Initially discovered as an enzyme specific for the formation of pseudouridine at position 55 in the T-loop of archaeal tRNA (Roovers et al., 2006), it was later shown to also catalyze the formation of pseudouridine at position 54 (Gurha & Gupta 2008). Pus10 is universally found in Archaea and only in few Eukarya, however in this last case the real function of eukaryal Pus10 in still unknown.
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
U:Y
RNA
tRNA
55
CCA
CCA
tRNATrp UCC
TΨC-loop
Archaeal Cytosol
18952823
U:Y
RNA
tRNA
54
CCA
CCA
tRNATrp CCA
TΨC-loop
Archaeal Cytosol
18952823
Alpha Fold Predicted Structure:
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Protein sequence:
M
I
L
E
K
A
R
E
I
L
E
E
H
Q
L
C
N
H
C
L
G
R
L
F
G
K
L
G
K
G
T
N
E
E
R
G
R
A
I
R
L
L
L
S
M
E
T
G
K
E
Y
K
E
P
E
K
C
E
L
C
G
G
V
F
N
N
L
D
K
F
A
E
L
C
I
K
A
A
E
G
I
E
F
E
T
F
W
V
G
S
R
F
P
E
E
I
E
K
K
E
E
E
I
W
R
K
F
R
V
V
S
G
E
K
I
T
K
E
F
N
R
E
L
G
K
V
I
A
V
R
Y
G
K
T
P
V
K
E
R
P
D
V
V
F
I
V
E
P
F
S
E
K
V
E
L
Q
V
N
P
I
Y
V
A
G
R
Y
R
K
L
I
R
G
I
P
Q
T
P
A
P
G
F
K
E
S
I
A
T
I
I
C
R
A
F
K
K
H
F
H
G
K
C
I
F
K
G
A
G
R
E
D
V
D
V
R
M
L
G
N
G
R
P
F
V
V
E
I
K
R
P
R
K
R
K
V
N
L
K
D
I
E
E
E
I
N
Q
S
G
K
V
E
V
L
N
L
R
F
I
T
P
E
E
A
E
R
I
L
T
T
R
H
R
K
V
Y
E
A
I
V
Y
V
K
D
G
I
T
K
E
E
V
E
K
V
V
K
S
L
K
N
A
E
I
K
Q
R
T
P
R
R
V
L
N
S
R
A
D
L
V
R
V
R
K
V
Y
D
V
K
G
E
L
I
D
D
K
H
F
K
L
R
L
V
T
D
G
G
L
Y
I
K
E
L
I
S
G
D
R
G
R
T
T
P
S
V
S
E
I
L
G
K
E
A
W
C
E
I
L
D
V
L
E
V
L
D
D
V
E
G
D
N
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
Formation of the conserved pseudouridine at position 55 in archaeal tRNA.
Roovers M, Hale C, Tricot C, Terns MP, Terns RM, Grosjean H, Droogmans L
Nucleic Acids Res
[details]
16920741
-
Archaeal Pus10 proteins can produce both pseudouridine 54 and 55 in tRNA.
Gurha P, Gupta R
RNA
[details]
18952823
-
tRNA binding, positioning and modification by the pseudouridine synthase Pus10.
Kamalampeta R, Keffer-Wilkes LC, Kothe U...
J Mol Biol
[details]
23743107
-