Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA N6-threonylcarbamoyladenosine(37) synthesis protein
Synonym: YjeE
GI: 16131990
COG: COG0802
UniProt: P0AF67
Alpha Fold Predicted Structure: AF-P0AF67-F1
Complex: TsaBDE
Enzyme type: ATPase
Position of modification - modification: t:37 - t6A



Protein sequence:

MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTFSRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFANDAICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG

Comments:

This protein belongs to P-loop ATPases. It binds ATP and displays a weak ATPase activity (produce ADP). This protein tends to oligomerize in vitro. Despite TsaC (YrdC), TsaD (YgjD) and TsaB (YeaZ) are present in all bacteria, TsaE (YjeE) is absent in several unicellular and symbiotic bacteria, suggesting that some bacteria can carry out t6A synthesis in absence of YjeE. However, TsaD, TsaC, TsaE and TsaB are both necessary and sufficient for t6A biosynthesis in vitro.





Alpha Fold Predicted Structure:






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Protein sequence:

M M N R V I P L P D E Q A T L D L G E R V A K A C D G A T V I Y L Y G D L G A G K T T F S R G F L Q A L G H Q G N V K S P T Y T L V E P Y T L D N L M V Y H F D L Y R L A D P E E L E F M G I R D Y F A N D A I C L V E W P Q Q G T G V L P D P D V E I H I D Y Q A Q G R E A R V S A V S S A G E L L L A R L A G

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P0AF67-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P0AF67-F1.cif  
DSSP Secondary Structures   P0AF67.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Biosynthesis of threonylcarbamoyl adenosine (t6A), a universal tRNA nucleoside. Deutsch C, El Yacoubi B, de Crecy-Lagard V, Iwata-Reuyl D J Biol Chem [details] 22378793 -
Effects on transcription of mutations in ygjD, yeaZ, and yjeE genes, which are involved in a universal tRNA modification in Escherichia coli. Hashimoto C, Sakaguchi K, Taniguchi Y, Honda H, Oshima T, Ogasawara N, Kato J J Bacteriol [details] 21873492 -
Known bioactive small molecules probe the function of a widely conserved but enigmatic bacterial ATPase, YjeE. Mangat CS, Brown ED Chem Biol [details] 19101473 -
Conserved P-loop GTPases of unknown function in bacteria: an emerging and vital ensemble in bacterial physiology. Brown ED Biochem Cell Biol [details] 16333325 -
Mechanism of N6-threonylcarbamoyladenonsine (t(6)A) biosynthesis: isolation and characterization of the intermediate threonylcarbamoyl-AMP. Lauhon CT... Biochemistry [details] 23072323 -