Full name: AdoMet-dependent rRNA methyltransferase SPB1
Synonym: YCL54W, YCL431
GI: 6226708
Orf: YCL054W
COG: COG0293
UniProt: P25582
Structures: | |
Complex:
Enzyme type: methyltransferase
Position of modification - modification: l:2922(2551) - Gm

Comments:

Spb1 methylates the conserved G2922 (2551 in E.coli numbering) in the so-called A-loop (hairpin 92) of the peptidyltransferase center (domain V) in cytoplasmic pre-rRNA of large subunit rRNA into Gm. It also methylates U2921 (2552 in E. coli numbering) into Um, but only when snR52 guide RNA has been disrupted, otherwise U2921 is normally methylated within the nucleolus by Nop1 (snoRNP machinery). AdoMet is the methyl group donor. The human homolog has also been identified (FTSJ3).

Protein sequence:

MGKTQKKNSKGRLDRYYYLAKEKGYRARSSFKIIQINEKYGHFLEKSKVVIDLCAAPGSWCQVASKLCPV NSLIIGVDIVPMKPMPNVITFQSDITTEDCRSKLRGYMKTWKADTVLHDGAPNVGLGWVQDAFTQSQLTL QALKLAVENLVVNGTFVTKIFRSKDYNKLIWVFQQLFEKVEATKPPASRNVSAEIFVVCKGFKAPKRLDP RLLDPKEVFEELPDGQQNMESKIYNPEKKVRKRQGYEEGDNLLYHETSILDFVRTEDPISMLGEMNKFTI DENDHEWKILKKLKQTTDEFRSCIEDLKVLGKKDFKMILRWRKIAREILGIEVKDDAKTEIEVVPLTEEE QIEKDLQGLQEKQRLNVKRERRRKNEMKQKELQRMQMNMITPTDIGIEAASLGKESLFNLKTAEKTGILN DLAKGKKRMIFTDDELAKDNDIYIDENIMIKDKDSAADADDLESELNAMYSDYKTRRSERDAKFRAKQAR GGDNEEEWTGFNEGSLEKKEEEGKDYIEDNDDEGVEGDSDDDEAITNLISKLKGQEGDHKLSSKARMIFN DPIFNNVEPDLPVNTVNDGIMSSESVGDISKLNKKRKHEEMHQKQDEADSSDESSSDDSDFEIVANDNAS EEFDSDYDSEEEKNQTKKEKHSRDIDIATVEAMTLAHQLALGQKNKHDLVDEGFNRYTFRDTENLPDWFL EDEKEHSKINKPITKEAAMAIKEKIKAMNARPIKKVAEAKARKRMRAVARLEKIKKKAGLINDDSDKTEK DKAEEISRLMRKVTKKPKTKPKVTLVVASGRNKGLAGRPKGVKGKYKMVDGVMKNEQRALRRIAKKHHKK K

Enzymatic activities:

Reaction Substrate Type Position
G:Gm rRNA (r) all/27S/prokaryotic cytosol 2922
U:Um rRNA (r) all/27S/prokaryotic cytosol 2921

Publications:

Title Authors Journal Details PubMed Id DOI
Spb1p-directed formation of Gm2922 in the ribosome catalytic center occurs at a late processing stage. Lapeyre B, Purushothaman SK Mol Cell [details] 15546625 -
Functional redundancy of Spb1p and a snR52-dependent mechanism for the 2'-O-ribose methylation of a conserved rRNA position in yeast. Bonnerot C, Pintard L, Lutfalla G Mol Cell [details] 14636587 -
Spb1p is a putative methyltransferase required for 60S ribosomal subunit biogenesis in Saccharomyces cerevisiae. Kressler D, Rojo M, Linder P, Cruz J Nucleic Acids Res [details] 10556316 -
Spb1p is a yeast nucleolar protein associated with Nop1p and Nop58p that is able to bind S-adenosyl-L-methionine in vitro. Pintard L, Kressler D, Lapeyre B Mol Cell Biol [details] 10648622 -
Copyright © Genesilico - All rights reserved
If you have any advice or suggestions for corrections or improvements, please contact: Pietro Boccaletto