Modomics - A Database of RNA Modifications

Full name:	tRNA pseudouridine(38,39,40) synthase
Synonym:	HisT, AsuC, HisT, LeuK
GI:	123176
Orf:	b2318
COG:	COG0101
UniProt:	P07649
Structures:	\| 1DJ0 \| 2NQP \| 2NR0 \| 2NRE \|
Complex:
Enzyme type:	pseudouridine synthase
Position of modification - modification:	t:38 - Y t:39 - Y t:40 - Y

Comments:

Region specific.

Protein sequence:

MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALSQVANEPITVFCAGRTDAGVHGTGQVVHFETTALRKDAAWTLGVNANLPGDIAVRWV
KTVPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGENDFTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFV
HHMVRNIVGSLMEVGAHNQPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD

Enzymatic activities:

Reaction	Substrate	Type	Position
U:Y	tRNA (t)	Gln/UUG/prokaryotic cytosol	38
U:Y	tRNA (t)	Gln/CUG/prokaryotic cytosol	38
U:Y	tRNA (t)	His/GUG/prokaryotic cytosol	38
U:Y	tRNA (t)	Leu/CAG/prokaryotic cytosol	38
U:Y	tRNA (t)	Leu/GAG/prokaryotic cytosol	38
U:Y	tRNA (t)	Asn/GUU/prokaryotic cytosol	39
U:Y	tRNA (t)	Cys/GCA/prokaryotic cytosol	39
U:Y	tRNA (t)	Gln/CUG/prokaryotic cytosol	39
U:Y	tRNA (t)	His/GUG/prokaryotic cytosol	39
U:Y	tRNA (t)	Ile/CAU/prokaryotic cytosol	39
U:Y	tRNA (t)	Leu/UAA/prokaryotic cytosol	39
U:Y	tRNA (t)	Lys/SUU/prokaryotic cytosol	39
U:Y	tRNA (t)	Met/CAU/prokaryotic cytosol	39
U:Y	tRNA (t)	Phe/GAA/prokaryotic cytosol	39
U:Y	tRNA (t)	Tyr/GUA/prokaryotic cytosol	39
U:Y	tRNA (t)	Arg/UCU/prokaryotic cytosol	40
U:Y	tRNA (t)	Leu/CAG/prokaryotic cytosol	40
U:Y	tRNA (t)	Ser/GGA/prokaryotic cytosol	40

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I.	Kammen HO, Marvel CC, Hardy L, Penhoet EE	J Biol Chem	[details]	3276686	-
Pre-steady-state kinetic analysis of the three Escherichia coli pseudouridine synthases TruB, TruA, and RluA reveals uniformly slow catalysis.	Wright JR, Keffer-Wilkes LC, Dobing SR, Kothe U	RNA	[details]	21998096	-