Modomics - A Database of RNA Modifications

ID Card:

Full name: SIT4-associating protein SAP185
GI: 6322363
Orf: J0840
COG: COG2253
UniProt: P40856
Alpha Fold Predicted Structure: AF-P40856-F1
Enzyme type: thiolase



Protein sequence:

MSGSFWKFGQDFGSQSPLAKLLNRAFIKIDDKPTSTEAGKIDSNSTDESLESNSFKSEDEEEEYELPNREEDYKAYKPNLSLLNDLLDDEELYTELMCSNFKLLVYLKYPEVLSKLIDYVRNSTILESNIDRVTSEDRDLVRGEDKDTTEDFENAKADKKNIDGTFEEKERTRSGEEEELENEENDSASEDTRVTLPHELEEHDDTRRARIAAEILSADVWPISSALIENEGLLAKLWSILRLPSPLSIEASTYFMKINERLLDMNMDGIIEFILKKEHIVDDFLAHIDNPPLMDFLLKVISTDKPEISNGVIQLFKKQNLVPKLIHLLDPVFDSCTQSAAGDFLKALVTISGNCPNEITSSIGPNELTRQLVSPNMMKQLMDIMLKGGNSLNNGVGIIIELIRKNNSDYDTIQTNYTTIESHPPTDRDPIYLGYLVKMFSEHMADFNKILTEKKIPLLQTSYGTIEPLGFERFKICELIAELLHCSNMTLLNEPSAYDIVRERDAERERIFNSQNYVDSNDRSELKENEDDNTGDADDEVEDDTNQVESANTSIDGEEVIDKLNSLQIETNKVNQNMNNEEQHSLMPDFNNGDFKDEEDENPFEPQYSDVILDSSDIEKNFRVSPNVGDQLKISLQDTRVIDTMLEMFFHFQWNNFLHNVVYDVVQQIFNGPLKIGYNRFLLDDLLINIRLTDMIINGNNECIEYEKGHDTRLGYMGHLTLIAEEVTKFTAYIEEMNITFENTEVMSSLFESKWIAYTEDVLEDLKEKYNAILGDIAEEGDMLQDEEEDAVYDKGERTMGTVDDYINDIMQMDNVRCQEEEEDEGEGYVSFDEDEPQEYRNGDSVRSKESNSSEGKRDQEQLYYEYVNEDGTKTRLNFNPDSDATEQVPGEVNRDHKIPLKLKRSFTDACKSETIPNNTVNAKEESVFQFSNELSDGWESSPSNSIPKRASPSKNGMNSPMFQHQFELHSPTDEFGGHKDEILSAEGHDYDIDEYDELSDDSDEEYDNCEDEDSLDYADSAAYALCRSKSKDKISWDEEEQARLMGVVKFNSEHYRD

Comments:

One of protein cofactors required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA. The enzyme that catalyzes the reaction is still unknown.





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M S G S F W K F G Q D F G S Q S P L A K L L N R A F I K I D D K P T S T E A G K I D S N S T D E S L E S N S F K S E D E E E E Y E L P N R E E D Y K A Y K P N L S L L N D L L D D E E L Y T E L M C S N F K L L V Y L K Y P E V L S K L I D Y V R N S T I L E S N I D R V T S E D R D L V R G E D K D T T E D F E N A K A D K K N I D G T F E E K E R T R S G E E E E L E N E E N D S A S E D T R V T L P H E L E E H D D T R R A R I A A E I L S A D V W P I S S A L I E N E G L L A K L W S I L R L P S P L S I E A S T Y F M K I N E R L L D M N M D G I I E F I L K K E H I V D D F L A H I D N P P L M D F L L K V I S T D K P E I S N G V I Q L F K K Q N L V P K L I H L L D P V F D S C T Q S A A G D F L K A L V T I S G N C P N E I T S S I G P N E L T R Q L V S P N M M K Q L M D I M L K G G N S L N N G V G I I I E L I R K N N S D Y D T I Q T N Y T T I E S H P P T D R D P I Y L G Y L V K M F S E H M A D F N K I L T E K K I P L L Q T S Y G T I E P L G F E R F K I C E L I A E L L H C S N M T L L N E P S A Y D I V R E R D A E R E R I F N S Q N Y V D S N D R S E L K E N E D D N T G D A D D E V E D D T N Q V E S A N T S I D G E E V I D K L N S L Q I E T N K V N Q N M N N E E Q H S L M P D F N N G D F K D E E D E N P F E P Q Y S D V I L D S S D I E K N F R V S P N V G D Q L K I S L Q D T R V I D T M L E M F F H F Q W N N F L H N V V Y D V V Q Q I F N G P L K I G Y N R F L L D D L L I N I R L T D M I I N G N N E C I E Y E K G H D T R L G Y M G H L T L I A E E V T K F T A Y I E E M N I T F E N T E V M S S L F E S K W I A Y T E D V L E D L K E K Y N A I L G D I A E E G D M L Q D E E E D A V Y D K G E R T M G T V D D Y I N D I M Q M D N V R C Q E E E E D E G E G Y V S F D E D E P Q E Y R N G D S V R S K E S N S S E G K R D Q E Q L Y Y E Y V N E D G T K T R L N F N P D S D A T E Q V P G E V N R D H K I P L K L K R S F T D A C K S E T I P N N T V N A K E E S V F Q F S N E L S D G W E S S P S N S I P K R A S P S K N G M N S P M F Q H Q F E L H S P T D E F G G H K D E I L S A E G H D Y D I D E Y D E L S D D S D E E Y D N C E D E D S L D Y A D S A A Y A L C R S K S K D K I S W D E E E Q A R L M G V V K F N S E H Y R D

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P40856-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P40856-F1.cif  
DSSP Secondary Structures   P40856.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. Huang B, Lu J, Bystrom AS RNA [details] 18755837 -