Modomics - A Database of RNA Modifications

ID Card:

Full name: Thiosulfate sulfurtransferase TUM1
GI: 51012915
COG: COG2897
UniProt: Q08686
Structures: | 3UTN |
Alpha Fold Predicted Structure: AF-Q08686-F1
Enzyme type: thiosulfate sulfurtransferase


PDB Structures:


3UTN

Structure Description:

Title: Crystal structure of Tum1 protein from Saccharomyces cerevisiae
Classification: TRANSFERASE
Technique: X-Ray Diffraction
Resolution: 1.90
R value free: 0.228
R value observed: 0.205
R value work: 0.204

Abstract of the PDB Structure's related Publication:

Yeast tRNA-thiouridine modification protein 1 (Tum1) plays essential role in the sulfur transfer process of Urm1 system, which in turn is involved in many important cellular processes. In the rhodanese-like domain (RLD), conserved cysteine residue is proved to be the centre of active site of sulfurtransferases and crucial for the substrate recognition. In this report, we describe the crystal structure of Tum1 protein at 1.90 A resolution which, despite consisting of two RLDs, has only one conserved cysteine residue in the C-terminal RLD. An unaccounted electron density is found near the active site, which might point to the new cofactor in the sulfur transfer mechanism.

Download RCSB-PDB Structures:

Pdb Files   3UTN.pdb   3V8V.pdb  
Pdbx/mmCIF Files   3UTN.cif   3V8V.cif  


Protein sequence:

MPLFDLISPKAFVKLVASEKVHRIVPVDATWYLPSWKLDNKVDFLTKPRIPNSIFFDIDAISDKKSPYPHMFPTKKVFDDAMSNLGVQKDDILVVYDRVGNFSSPRCAWTLGVMGHPKVYLLNNFNQYREFKYPLDSSKVAAFSPYPKSHYESSESFQDKEIVDYEEMFQLVKSGELAKKFNAFDARSLGRFEGTEPEPRSDIPSGHIPGTQPLPYGSLLDPETKTYPEAGEAIHATLEKALKDFHCTLDPSKPTICSCGTGVSGVIIKTALELAGVPNVRLYDGSWTEWVLKSGPEWIAENRD

Comments:

Part of eukaryotic sulfur-relay system required for in 2-thiolation of mcm5S2U at tRNA wobble positions.





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M P L F D L I S P K A F V K L V A S E K V H R I V P V D A T W Y L P S W K L D N K V D F L T K P R I P N S I F F D I D A I S D K K S P Y P H M F P T K K V F D D A M S N L G V Q K D D I L V V Y D R V G N F S S P R C A W T L G V M G H P K V Y L L N N F N Q Y R E F K Y P L D S S K V A A F S P Y P K S H Y E S S E S F Q D K E I V D Y E E M F Q L V K S G E L A K K F N A F D A R S L G R F E G T E P E P R S D I P S G H I P G T Q P L P Y G S L L D P E T K T Y P E A G E A I H A T L E K A L K D F H C T L D P S K P T I C S C G T G V S G V I I K T A L E L A G V P N V R L Y D G S W T E W V L K S G P E W I A E N R D
50100150200250300SequenceGHTBSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q08686-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q08686-F1.cif  
DSSP Secondary Structures   Q08686.dssp  





Publications: