Modomics - A Database of RNA Modifications

Full name:	RNA-capping enzyme
Synonym:	HCE, mRNA 5'-triphosphatase, GTP--RNA guanylyltransferase
GI:	6685628
Orf:	RNGTT
COG:	COG5226
UniProt:	O60942
Structures:	\| 3S24 \|
Complex:
Enzyme type:	triphosphatase, guanylyltransferase
Position of modification - modification:	m:0 - m7GpppN

Comments:

Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part.

Protein sequence:

MAHNKIPPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKVKMGLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTT
ENTETFIRLCERFNERNPPELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRYGDIEEAPPPPLLPDWCFEDDEDE
DEDEDGKKESEPGSSASFGKRRKERLKLGAIFLEGVTVKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIKLLDLKPYKVSWKADGTRY
MMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGL
IDKTQEPFSVRNKPFFDICTSRKLLEGNFAKEVSHEMDGLIFQPTGKYKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQ
IKVTKELKQYDNKIIECKFENNSWVFMRQRTDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCTAASQGQKRKHHLDPDTELMPPPPPKRPRPLT

Enzymatic activities:

Reaction	Substrate	Type	Position
pppN:ppN	mRNA (m)		0
ppN:GpppN	mRNA (m)		0

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Isolation and characterization of a human cDNA for mRNA 5'-capping enzyme.	Yamada-Okabe T, Doi R, Shimmi O, Arisawa M, Yamada-Okabe H	Nucleic Acids Res	[details]	9512541	-
Cloning and characterization of two human cDNAs encoding the mRNA capping enzyme.	Tsukamoto T, Shibagaki Y, Murakoshi T, Suzuki M, Nakamura A, Gotoh H, Mizumoto K	Biochem Biophys Res Commun	[details]	9473487	-
Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes.	Pillutla RC, Yue Z, Maldonado E, Shatkin AJ	J Biol Chem	[details]	9705270	-
Structure of the guanylyltransferase domain of human mRNA capping enzyme.	Chu C, Das K, Tyminski JR, Bauman JD, Guan R, Qiu W, Montelione GT, Arnold E, Shatkin AJ	Proc Natl Acad Sci U S A	[details]	21636784	-

Links:

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