RNMT catalyzes methylation of the cap at the N-7 position to create the methyl cap, m7G(5')ppp(5')X. RNMT can only catalyze methylation of guanosine when it is a component of a cap structure attached to a transcript. RAM (RNMT-Activating Mini protein)/Fam103a1 is required for efficient cap methylation in vitro and in vivo, and is indirectly required to maintain mRNA expression levels, for mRNA translation and for cell viability.
Characterization of human, Schizosaccharomyces pombe, and Candida albicans mRNA cap methyltransferases and complete replacement of the yeast capping apparatus by mammalian enzymes.
Cap methyltransferase selective binding and methylation of GpppG-RNA are stimulated by importin-alpha.
Characterization of human, Schizosaccharomyces pombe, and Candida albicans mRNA cap methyltransferases and complete replacement of the yeast capping apparatus by mammalian enzymes.
Cloning and characterization of three human cDNAs encoding mRNA (guanine-7-)-methyltransferase, an mRNA cap methylase.
Human mRNA cap methyltransferase: alternative nuclear localization signal motifs ensure nuclear localization required for viability.
RAM/Fam103a1 is required for mRNA cap methylation.
Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes.