Modomics - A Database of RNA Modifications

Full name:	cap methyltranferase 1
Synonym:	hMTr1, FTSJD2, KIAA0082
GI:	74750894
Orf:	FTSJD2, KIAA0082
COG:	COG0293
UniProt:	Q8N1G2
Structures:	\| \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	m:1 - m7GpppNm n:1 - m7GpppNm

Comments:

hMTr1 protein transfers a methyl group from S-adenosylmethionine to the 2'-O-ribose of the first nucleotide of messenger RNA and small nuclear RNA. N(7) methylation of the guanosine cap is not required for hMTr1 activity.

Protein sequence:

MKRRTDPECTAPIKKQKKRVAELALSLSSTSDDEPPSSVSHGAKASTTSLSGSDSETEGKQHSSDSFDDAFKADSLVEGTSSRYSMYNSVSQKLMAKMGF
REGEGLGKYSQGRKDIVEASSQKGRRGLGLTLRGFDQELNVDWRDEPEPSACEQVSWFPECTTEIPDTQEMSDWMVVGKRKMIIEDETEFCGEELLHSVL
QCKSVFDVLDGEEMRRARTRANPYEMIRGVFFLNRAAMKMANMDFVFDRMFTNPRDSYGKPLVKDREAELLYFADVCAGPGGFSEYVLWRKKWHAKGFGM
TLKGPNDFKLEDFYSASSELFEPYYGEGGIDGDGDITRPENISAFRNFVLDNTDRKGVHFLMADGGFSVEGQENLQEILSKQLLLCQFLMALSIVRTGGH
FICKTFDLFTPFSVGLVYLLYCCFERVCLFKPITSRPANSERYVVCKGLKVGIDDVRDYLFAVNIKLNQLRNTDSDVNLVVPLEVIKGDHEFTDYMIRSN
ESHCSLQIKALAKIHAFVQDTTLSEPRQAEIRKECLRLWGIPDQARVAPSSSDPKSKFFELIQGTEIDIFSYKPTLLTSKTLEKIRPVFDYRCMVSGSEQ
KFLIGLGKSQIYTWDGRQSDRWIKLDLKTELPRDTLLSVEIVHELKGEGKAQRKISAIHILDVLVLNGTDVREQHFNQRIQLAEKFVKAVSKPSRPDMNP
IRVKEVYRLEEMEKIFVRLEMKIIKGSSGTPKLSYTGRDDRHFVPMGLYIVRTVNEPWTMGFSKSFKKKFFYNKKTKDSTFDLPADSIAPFHICYYGRLF
WEWGDGIRVHDSQKPQDQDKLSKEDVLSFIQMHRA

Enzymatic activities:

Reaction	Substrate	Type	Position
m7GpppN:m7GpppNm	mRNA (m)		1
m7GpppN:m7GpppNm	snRNA (sn)	U1	1
m7GpppN:m7GpppNm	snRNA (sn)	U2//	1
GpppN:GpppNmN	mRNA (m)		1
GpppN:GpppNmN	snRNA (sn)	U1	1
GpppN:GpppNmN	snRNA (sn)	U2//	1

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Characterization of hMTr1, a human Cap1 2'-O-ribose methyltransferase.	Belanger F, Stepinski J, Darzynkiewicz E, Pelletier J	J Biol Chem	[details]	20713356	-
The human interferon-regulated ISG95 protein interacts with RNA polymerase II and shows methyltransferase activity.	Haline-Vaz T, Silva TC, Zanchin NI	Biochem Biophys Res Commun	[details]	18533109	-
Structural basis for m7G-cap hypermethylation of small nuclear, small nucleolar and telomerase RNA by the dimethyltransferase TGS1.	Monecke T, Dickmanns A, Ficner R	Nucleic Acids Res	[details]	19386620	-

Links:

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