Modomics - A Database of RNA Modifications

Full name:	cap methyltranferase 2
Synonym:	hMTr2, FTSJD1, FLJ11171
GI:	269849596
Orf:	FTSJD1, FLJ11171
COG:	COG0293
UniProt:	Q8IYT2
Structures:	\| \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	m:2 - m7GpppNmNm n:2 - m7GpppNmNm

Comments:

hMTr2 protein transfers a methyl group from S-adenosylmethionine to the 2'-O-ribose of the second nucleotide of messenger RNA and small nuclear RNA. Neither N(7) methylation of the guanosine cap nor 2'-O-ribose methylation of the first transcribed nucleotide are required for hMTr2, but the presence of cap1 methylation increases hMTr2 activity. The hMTr2 protein is distributed throughout the nucleus and cytosol, in contrast to the nuclear hMTr1.

Protein sequence:

MSKCRKTPVQQLASPASFSPDILADIFELFAKNFSYGKPLNNEWQLPDPSEIFTCDHTELNAFLDLKNSLNEVKNLLSDKKLDEWHEHTAFTNKAGKIIS
HVRKSVNAELCTQAWCKFHEILCSFPLIPQEAFQNGKLNSLHLCEAPGAFIASLNHYLKSHRFPCHWSWVANTLNPYHEANDDLMMIMDDRLIANTLHWW
YFGPDNTGDIMTLKFLTGLQNFISSMATVHLVTADGSFDCQGNPGEQEALVSSLHYCEVVTALTTLGNGGSFVLKMFTMFEHCSINLMYLLNCCFDQVHV
FKPATSKAGNSEVYVVCLHYKGREAIHPLLSKMTLNFGTEMKRKALFPHHVIPDSFLKRHEECCVFFHKYQLETISENIRLFECMGKAEQEKLNNLRDCA
IQYFMQKFQLKHLSRNNWLVKKSSIGCSTNTKWFGQRNKYFKTYNERKMLEALSWKDKVAKGYFNSWAEEHGVYHPGQSSILEGTASNLECHLWHILEGK
KLPKVKCSPFCNGEILKTLNEAIEKSLGGAFNLDSKFRPKQQYSCSCHVFSEELIFSELCSLTECLQDEQVVVPSNQIKCLLVGFSTLRNIKMHIPLEVR
LLESAELTTFSCSLLHDGDPTYQRLFLDCLLHSLRELHTGDVMILPVLSCFTRFMAGLIFVLHSCFRFITFVCPTSSDPLRTCAVLLCVGYQDLPNPVFR
YLQSVNELLSTLLNSDSPQQVLQFVPMEVLLKGALLDFLWDLNAAIAKRHLHFIIQREREEIINSLQLQN

Enzymatic activities:

Reaction	Substrate	Type	Position
m7GpppNm:m7GpppNmNm	mRNA (m)		2
m7GpppNm:m7GpppNmNm	snRNA (sn)	U1	2
m7GpppNm:m7GpppNmNm	snRNA (sn)	U2//	2
m7GpppN:m7GpppNNm	mRNA (m)		2
m7GpppN:m7GpppNNm	snRNA (sn)	U1	2
m7GpppN:m7GpppNNm	snRNA (sn)	U2//	2
GpppN:GpppNNm	mRNA (m)		2
GpppN:GpppNNm	snRNA (sn)	U1	2
GpppN:GpppNNm	snRNA (sn)	U2//	2

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
2'-O-ribose methylation of cap2 in human: function and evolution in a horizontally mobile family.	Werner M, Purta E, Kaminska KH, Cymerman IA, Campbell DA, Mittra B, Zamudio JR, Sturm NR, Jaworski J, Bujnicki JM	Nucleic Acids Res	[details]	21310715	-

Links:

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