ID Card:
Full name:
N6-adenosine-methyltransferase IME4
Synonym:
Mrm(m6A), Spo8
GI:
1174426
Orf:
YGL192W, G1337
COG:
COG4725
UniProt:
P41833
Alpha Fold Predicted Structure:
AF-P41833-F1
Enzyme type:
methyltransferase
Position of modification - modification:
m :many - m6A
Protein sequence:
MINDKLVHFLIQNYDDILRAPLSGQLKDVYSLYISGGYDDEMQKLRNDKDEVLQFEQFWNDLQDIIFATPQSIQFDQNLLVADRPEKIVYLDVFSLKILYNKFHAFYYTLKSSSSSCEEKVSSLTTKPEADSEKDQLLGRLLGVLNWDVNVSNQGLPREQLSNRLQNLLREKPSSFQLAKERAKYTTEVIEYIPICSDYSHASLLSTSVYIVNNKIVSLQWSKISACQENHPGLIECIQSKIHFIPNIKPQTDISLGDCSYLDTCHKLNTCRYIHYLQYIPSCLQERADRETASENKRIRSNVSIPFYTLGNCSAHCIKKALPAQWIRCDVRKFDFRVLGKFSVVIADPAWNIHMNLPYGTCNDIELLGLPLHELQDEGIIFLWVTGRAIELGKESLNNWGYNVINEVSWIKTNQLGRTIVTGRTGHWLNHSKEHLLVGLKGNPKWINKHIDVDLIVSMTRETSRKPDELYGIAERLAGTHARKLEIFGRDHNTRPGWFTIGNQLTGNCIYEMDVERKYQEFMKSKTGTSHTGTKKIDKKQPSKLQQQHQQQYWNNMDMGSGKYYAEAKQNPMNQKHTPFESKQQQKQQFQTLNNLYFAQ
Comments:
MIS complex (Mum2, Ime4, and Slz1) mediates N6-methyladenosine (m6A) methylation on some mRNAs during meiosis and
is required for sporulation, targeting 5'-[AG]GAC-3' consensus sites. Ime4 is the catalytic component of the MIS
complex (
Agarwala et al. 2012 ) and catalyses the AdoMet-dependent N6-methylation of internal adenosine in yeast mRNA, including mRNA coding for Ime4 (regulatory role).
Alpha Fold Predicted Structure:
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Protein sequence:
M
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Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene.
Clancy MJ, Shambaugh ME, Timpte CS, Bokar JA
Nucleic Acids Res
[details]
12384598
-
Yeast targets for mRNA methylation.
Bodi Z, Button JD, Grierson D, Fray RG
Nucleic Acids Res
[details]
20421205
-
RNA methylation by the MIS complex regulates a cell fate decision in yeast.
Agarwala SD, Blitzblau HG, Hochwagen A, Fink GR...
PLoS Genet
[details]
22685417
-
Links: