Modomics - A Database of RNA Modifications

ID Card:

Full name: rRNA cytydine C-5-methyltransferase
Synonym: kanamycin and tobramycin resistance 16S rRNA methyltransferase
GI: 383329552
Orf: efmM
UniProt: E9KIK3
Structures: | 3M4X |
Alpha Fold Predicted Structure: AF-E9KIK3-F1
Enzyme type: methyltransferase
Position of modification - modification: s:1404(1404) - m5C


PDB Structures:


3M4X

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:



Download RCSB-PDB Structures:

Pdb Files   3M4X.pdb  
Pdbx/mmCIF Files   3M4X.cif  


Protein sequence:

MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNPLKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAAAKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIVTNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILSSAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSVAGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFSNDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALATKKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFPKGLRFH

Comments:




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
C:m5C RNA rRNA 1404 LSU-23S Prokaryotic Cytosol 21159796   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M K E E A T T L P Q Q F I K K Y R L L L G E E A S D F F S A L E Q G S V K K G F R W N P L K P A G L D M V Q T Y H S E E L Q P A P Y S N E G F L G T V N G K S F L H Q A G Y E Y S Q E P S A M I V G T A A A A K P G E K V L D L C A A P G G K S T Q L A A Q M K G K G L L V T N E I F P K R A K I L S E N I E R W G V S N A I V T N H A P A E L V P H F S G F F D R I V V D A P C S G E G M F R K D P N A I K E W T E E S P L Y C Q K R Q Q E I L S S A I K M L K N K G Q L I Y S T C T F A P E E N E E I I S W L V E N Y P V T I E E I P L T Q S V S S G R S E W G S V A G L E K T I R I W P H K D Q G E G H F V A K L T F H G Q N Q M H K E K K T R K K S K V Q M T K E Q E K L W T E F S N D F H Y E A T G R L L V F N D H L W E V P E L A P S L D G L K V V R T G L H L G D F K K N R F E P S Y A L A L A T K K I E N I P C L P I T Q K E W Q S Y T A G E T F Q R D G N Q G W V L L V L D K I P V G F G K Q V K G T V K N F F P K G L R F H

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-E9KIK3-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-E9KIK3-F1.cif  
DSSP Secondary Structures   E9KIK3.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Intrinsic resistance to aminoglycosides in Enterococcus faecium is conferred by the 16S rRNA m5C1404-specific methyltransferase EfmM. Galimand M, Schmitt E, Panvert M, Desmolaize B, Douthwaite S, Mechulam Y, Courvalin P RNA [details] 21159796 -