Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA (cytosine(34)-C(5))-methyltransferase
Synonym: hMisu, SAKI
GI: 39995082
Orf: NSUN2
COG: COG0144
UniProt: Q08J23
Alpha Fold Predicted Structure: AF-Q08J23-F1
Enzyme type: methyltransferase
Position of modification - modification: pre-tRNA:34 - m5C
m:many - m5C
other:many - m5C
t:48 - m5C
t:49 - m5C
t:50 - m5C
pre-tRNA:48 - m5C



Protein sequence:

MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR

Comments:

Multisite specific enzyme. Orthologue of yeast Trm4. It modifies not only tRNA but also RPPH1 subunit of RNase P and some mRNAs. hTrm4 partially complements the lack of the endogenous SceTrm4p. Exists in two isoforms.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
C:m5C pre-tRNA (pre-t) Leu/CAA/prokaryotic cytosol 34 17071714   
C:m5C mRNA (m) 22344696   
C:m5C other (o) 22344696   
C:m5C tRNA (t) Leu/CAA/prokaryotic cytosol 48 22995836   
C:m5C pre-tRNA (pre-t) Leu/CAA/prokaryotic cytosol 48 22995836   
C:m5C tRNA (t) Gly/GCC/eukaryotic cytosol 48 22995836   
C:m5C tRNA (t) Gly/GCC/eukaryotic cytosol 49 22995836   
C:m5C tRNA (t) Gly/GCC/unknown 50 22995836   

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M G R R S R G R R L Q Q Q Q R P E D A E D G A E G G G K R G E A G W E G G Y P E I V K E N K L F E H Y Y Q E L K I V P E G E W G Q F M D A L R E P L P A T L R I T G Y K S H A K E I L H C L K N K Y F K E L E D L E V D G Q K V E V P Q P L S W Y P E E L A W H T N L S R K I L R K S P H L E K F H Q F L V S E T E S G N I S R Q E A V S M I P P L L L N V R P H H K I L D M C A A P G S K T T Q L I E M L H A D M N V P F P E G F V I A N D V D N K R C Y L L V H Q A K R L S S P C I M V V N H D A S S I P R L Q I D V D G R K E I L F Y D R I L C D V P C S G D G T M R K N I D V W K K W T T L N S L Q L H G L Q L R I A T R G A E Q L A E G G R M V Y S T C S L N P I E D E A V I A S L L E K S E G A L E L A D V S N E L P G L K W M P G I T Q W K V M T K D G Q W F T D W D A V P H S R H T Q I R P T M F P P K D P E K L Q A M H L E R C L R I L P H H Q N T G G F F V A V L V K K S S M P W N K R Q P K L Q G K S A E T R E S T Q L S P A D L T E G K P T D P S K L E S P S F T G T G D T E I A H A T E D L E N N G S K K D G V C G P P P S K K M K L F G F K E D P F V F I P E D D P L F P P I E K F Y A L D P S F P R M N L L T R T T E G K K R Q L Y M V S K E L R N V L L N N S E K M K V I N T G I K V W C R N N S G E E F D C A F R L A Q E G I Y T L Y P F I N S R I I T V S M E D V K I L L T Q E N P F F R K L S S E T Y S Q A K D L A K G S I V L K Y E P D S A N P D A L Q C P I V L C G W R G K A S I R T F V P K N E R L H Y L R M M G L E V L G E K K K E G V I L T N E S A A S T G Q P D N D V T E G Q R A G E P N S P D A E E A N S P D V T A G C D P A G V H P P R

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q08J23-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q08J23-F1.cif  
DSSP Secondary Structures   Q08J23.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Identification of human tRNA:m5C methyltransferase catalysing intron-dependent m5C formation in the first position of the anticodon of the pre-tRNA Leu (CAA). Brzezicha B, Schmidt M, Makalowska I, Jarmolowski A, Pienkowska J, Szweykowska-Kulinska Z... Nucleic Acids Res [details] 17071714 -
Widespread occurrence of 5-methylcytosine in human coding and non-coding RNA. Squires JE, Patel HR, Nousch M, Sibbritt T, Humphreys DT, Parker BJ, Suter CM, Preiss T... Nucleic Acids Res [details] 22344696 -
Function and detection of 5-methylcytosine in eukaryotic RNA. Squires JE, Preiss T... Epigenomics [details] 22122054 -
The Human tRNA m ( 5) C methyltransferase Misu is multisite-specific. Auxilien S, Guerineau V, Szweykowska-Kulinska Z, Golinelli-Pimpaneau B... RNA Biol [details] 22995836 -
Methylation by NSun2 represses the levels and function of microRNA 125b. Yuan S, Tang H, Xing J, Fan X, Cai X, Li Q, Han P, Luo Y, Zhang Z, Jiang B, Dou Y, Gorospe M, Wang W... Mol Cell Biol [details] 25047833 -

Links:

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