| Full name: | tRNA (cytosine(34)-C(5))-methyltransferase |
|---|---|
| Synonym: | hMisu, SAKI |
| GI: | 39995082 |
| Orf: | NSUN2 |
| COG: | COG0144 |
| UniProt: | Q08J23 |
| Structures: | | | |
| Complex: | |
| Enzyme type: | methyltransferase |
| Position of modification - modification: |
pre-tRNA:34 - m5C m:many - m5C other:many - m5C t:48 - m5C t:49 - m5C t:50 - m5C pre-tRNA:48 - m5C |
MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFK ELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHA DMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQL RIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPKDPEK LQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDG VCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCA FRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLR MMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR
| Reaction | Substrate | Type | Position |
|---|---|---|---|
| C:m5C | pre-tRNA (pre-t) | Leu/CAA/prokaryotic cytosol | 34 |
| C:m5C | mRNA (m) | ||
| C:m5C | other (o) | ||
| C:m5C | tRNA (t) | Leu/CAA/prokaryotic cytosol | 48 |
| C:m5C | pre-tRNA (pre-t) | Leu/CAA/prokaryotic cytosol | 48 |
| C:m5C | tRNA (t) | Gly/GCC/eukaryotic cytosol | 48 |
| C:m5C | tRNA (t) | Gly/GCC/eukaryotic cytosol | 49 |
| C:m5C | tRNA (t) | Gly/GCC/unknown | 50 |
| Title | Authors | Journal | Details | ||
|---|---|---|---|---|---|
| Identification of human tRNA:m5C methyltransferase catalysing intron-dependent m5C formation in the first position of the anticodon of the pre-tRNA Leu (CAA). | Brzezicha B, Schmidt M, Makalowska I, Jarmolowski A, Pienkowska J, Szweykowska-Kulinska Z... | Nucleic Acids Res | [details] | 17071714 | - |
| Widespread occurrence of 5-methylcytosine in human coding and non-coding RNA. | Squires JE, Patel HR, Nousch M, Sibbritt T, Humphreys DT, Parker BJ, Suter CM, Preiss T... | Nucleic Acids Res | [details] | 22344696 | - |
| Function and detection of 5-methylcytosine in eukaryotic RNA. | Squires JE, Preiss T... | Epigenomics | [details] | 22122054 | - |
| The Human tRNA m ( 5) C methyltransferase Misu is multisite-specific. | Auxilien S, Guerineau V, Szweykowska-Kulinska Z, Golinelli-Pimpaneau B... | RNA Biol | [details] | 22995836 | - |
| Methylation by NSun2 represses the levels and function of microRNA 125b. | Yuan S, Tang H, Xing J, Fan X, Cai X, Li Q, Han P, Luo Y, Zhang Z, Jiang B, Dou Y, Gorospe M, Wang W... | Mol Cell Biol | [details] | 25047833 | - |
| _PubMed_ |