| Full name: |
tRNA (guanine(37)-N1)-methyltransferase |
| Synonym: |
KIAA1393, TRMT5 |
| GI: |
145275187 |
| Orf: |
|
| COG: |
COG2520 |
| UniProt: |
Q32P41 |
| Structures: |
|
|
|
| Complex: |
|
| Enzyme type: |
methyltransferase |
| Position of modification - modification: |
t:37 - m1G
t:37 - o2yW
|
Comments:
The activity was studied using yeast tRNAPhe and E. coli tRNALeu. It was shown to catalyze also I->m1I reaction in position 37 in E.coli tRNAAsp hence it is predicted to participate in the formation of m1I in position 37 of human tRNAAla.
Protein sequence:
MVLWILWRPFGFSGRFLKLESHSITESKSLIPVAWTSLTQMLLEAPGIFLLGQRKRFSTMPETETHERET
ELFSPPSDVRGMTKLDRTAFKKTVNIPVLKVRKEIVSKLMRSLKRAALQRPGIRRVIEDPEDKESRLIML
DPYKIFTHDSFEKAELSVLEQLNVSPQISKYNLELTYEHFKSEEILRAVLPEGQDVTSGFSRIGHIAHLN
LRDHQLSFKHLIGQVMIDKNPGITSAVNKINNIDNMYRNFQMEVLSGEQNMMTKVRENNYTYEFDFSKVY
WNPRLSTEHSRITELLKPGDVLFDVFAGVGPFAIPVAKKNCTVFANDLNPESHKWLLYNCKLNKVDQKVK
VFNLDGKDFLQGPVKEELMQLLGLSKERKPSVHVVMNLPAKAIEFLSAFKWLLDGQPCSSEFLPIVHCYS
FSKDANPAEDVRQRAGAVLGISLEACSSVHLVRNVAPNKEMLCITFQIPASVLYKNQTRNPENHEDPPLK
RQRTAEAFSDEKTQIVSNT
Enzymatic activities:
| Reaction
| Substrate
| Type
| Position
|
|
G:m1G
|
tRNA (t) |
Phe/#AA/unknown |
37 |
|
G:m1G
|
tRNA (t) |
Leu/.AA/unknown |
37 |
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| Isolation and characterization of the human tRNA-(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein. |
Brule H, Elliott M, Redlak M, Zehner ZE, Holmes WM |
Biochemistry |
[details]
|
15248782
|
-
|
| Conservation of structure and mechanism by Trm5 enzymes. |
Christian T, Gamper H, Hou YM... |
RNA |
[details]
|
23887145
|
-
|
Links: