Modomics - A Database of RNA Modifications

Full name:	tRNA (guanine(37)-N1)-methyltransferase
Synonym:	KIAA1393, TRMT5
GI:	145275187
Orf:
COG:	COG2520
UniProt:	Q32P41
Structures:	\| \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	t:37 - m1G t:37 - o2yW

Comments:

The activity was studied using yeast tRNAPhe and E. coli tRNALeu. It was shown to catalyze also I->m1I reaction in position 37 in E.coli tRNAAsp hence it is predicted to participate in the formation of m1I in position 37 of human tRNAAla.

Protein sequence:

MVLWILWRPFGFSGRFLKLESHSITESKSLIPVAWTSLTQMLLEAPGIFLLGQRKRFSTMPETETHERETELFSPPSDVRGMTKLDRTAFKKTVNIPVLK
VRKEIVSKLMRSLKRAALQRPGIRRVIEDPEDKESRLIMLDPYKIFTHDSFEKAELSVLEQLNVSPQISKYNLELTYEHFKSEEILRAVLPEGQDVTSGF
SRIGHIAHLNLRDHQLSFKHLIGQVMIDKNPGITSAVNKINNIDNMYRNFQMEVLSGEQNMMTKVRENNYTYEFDFSKVYWNPRLSTEHSRITELLKPGD
VLFDVFAGVGPFAIPVAKKNCTVFANDLNPESHKWLLYNCKLNKVDQKVKVFNLDGKDFLQGPVKEELMQLLGLSKERKPSVHVVMNLPAKAIEFLSAFK
WLLDGQPCSSEFLPIVHCYSFSKDANPAEDVRQRAGAVLGISLEACSSVHLVRNVAPNKEMLCITFQIPASVLYKNQTRNPENHEDPPLKRQRTAEAFSD
EKTQIVSNT

Enzymatic activities:

Reaction	Substrate	Type	Position
G:m1G	tRNA (t)	Phe/#AA/unknown	37
G:m1G	tRNA (t)	Leu/.AA/unknown	37

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Isolation and characterization of the human tRNA-(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein.	Brule H, Elliott M, Redlak M, Zehner ZE, Holmes WM	Biochemistry	[details]	15248782	-
Conservation of structure and mechanism by Trm5 enzymes.	Christian T, Gamper H, Hou YM...	RNA	[details]	23887145	-

Links:

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