Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA (guanine(26)-N(2))-dimethyltransferase, mitochondrial
GI: 136242
Orf: YDR120C
COG: COG1867
UniProt: P15565
Alpha Fold Predicted Structure: AF-P15565-F1
Enzyme type: methyltransferase
Position of modification - modification: t:26 - m2,2G



Protein sequence:

MEGFFRIPLKRANLHGMLKAAISKIKANFTAYGAPRINIEDFNIVKEGKAEILFPKKETVFYNPIQQFNRDLSVTCIKAWDNLYGEECGQKRNNKKSKKKRCAETNDDSSKRQKMGNGSPKEAVGNSNRNEPYINILEALSATGLRAIRYAHEIPHVREVIANDLLPEAVESIKRNVEYNSVENIVKPNLDDANVLMYRNKATNNKFHVIDLDPYGTVTPFVDAAIQSIEEGGLMLVTCTDLSVLAGNGYPEKCFALYGGANMVSHESTHESALRLVLNLLKQTAAKYKKTVEPLLSLSIDFYVRVFVKVKTSPIEVKNVMSSTMTTYHCSRCGSYHNQPLGRISQREGRNNKTFTKYSVAQGPPVDTKCKFCEGTYHLAGPMYAGPLHNKEFIEEVLRINKEEHRDQDDTYGTRKRIEGMLSLAKNELSDSPFYFSPNHIASVIKLQVPPLKKVVAGLGSLGFECSLTHAQPSSLKTNAPWDAIWYVMQKCDDEKKDLSKMNPNTTGYKILSAMPGWLSGTVKSEYDSKLSFAPNEQSGNIEKLRKLKIVRYQENPTKNWGPKARPNTS

Comments:

S-Adenosyl methionine (SAM or AdoMet) consists of an adenosyl cation attached to the sulfur methionine. It is synthesized from ATP and methionine by S-Adenosylmethionine synthetase enzyme through the the reaction of ATP with L-methionine and water forming as catalytical product S-adenosyl-L-methionine. SAM is a common cosubstrate involved in several reactions, among which methyl group transfers. Trm1 di-methylates a single guanine residue at position 26 of most tRNAs using SAM as a donor of the methyl groups, and is required for the modification of both mitochondrial and cytoplasmic tRNAs.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
G:m2G RNA tRNA 26 CAC CAC tRNAValCAC ~ Mitochondrion and Cytoplasm 2426253   
G:m2G RNA tRNA 26 UGG UGG tRNAProUGG ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 UGA UGA tRNASerUGA ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 UAG UAG tRNAThrUAG ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 GUA GUA tRNATyrGUA ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 IGC IGC tRNAAlaIGC ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 IGC IGC tRNAAlaIGC ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 ICU ICU tRNAArgICU ~ Mitochondrion and Cytoplasm
m2G:m2,2G tRNA (t) tRNA 26 ICU ICU tRNAArgICU ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 ICG ICG tRNAArgICG ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 ICG ICG tRNAArgICG ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 GUU GUU tRNAAsnGUU ~ ~
m2G:m2,2G RNA tRNA 26 GUU GUU tRNAAsnGUU ~ Mitochondrion and Cytoplasm
G:m2G RNA rRNA 26 UAG UAG tRNALeuUAG ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 UAG UAG tRNALeuUAG ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 CAA CAA tRNALeuCAA ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 CAA CAA tRNALeuCAA ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 UAA UAA tRNALeuUAA ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 UAA UAA tRNALeuUAA ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 CUU CUU tRNALysCUU ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 CUU CUU tRNALysCUU ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 SUU SUU tRNALysSUU ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 SUU SUU tRNALysSUU ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 CAU CAU tRNAMetCAU ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 CAU CAU tRNAMetCAU ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 GAA GAA tRNAPheGAA ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 GAA GAA tRNAPheGAA ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 CGA CGA tRNASerCGA ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 CGA CGA tRNASerCGA ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 UGA UGA tRNASerUGA ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 UGA UGA tRNASerUGA ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 IGA IGA tRNASerIGA ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 IGA IGA tRNASerIGA ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 IGU IGU tRNAThrIGU ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 IGU IGU tRNAThrIGU ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 GUA GUA tRNATyrGUA ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 GUA GUA tRNATyrGUA ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 CAC CAC tRNAValCAC ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 CAC CAC tRNAValCAC ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 UAA UAA tRNALeuUAA ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 UAA UAA tRNALeuUAA ~ Mitochondrion and Cytoplasm
G:m2G RNA tRNA 26 GAA GAA tRNAPheGAA ~ Mitochondrion and Cytoplasm
m2G:m2,2G RNA tRNA 26 GAA GAA tRNAPheGAA ~ Mitochondrion and Cytoplasm

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M E G F F R I P L K R A N L H G M L K A A I S K I K A N F T A Y G A P R I N I E D F N I V K E G K A E I L F P K K E T V F Y N P I Q Q F N R D L S V T C I K A W D N L Y G E E C G Q K R N N K K S K K K R C A E T N D D S S K R Q K M G N G S P K E A V G N S N R N E P Y I N I L E A L S A T G L R A I R Y A H E I P H V R E V I A N D L L P E A V E S I K R N V E Y N S V E N I V K P N L D D A N V L M Y R N K A T N N K F H V I D L D P Y G T V T P F V D A A I Q S I E E G G L M L V T C T D L S V L A G N G Y P E K C F A L Y G G A N M V S H E S T H E S A L R L V L N L L K Q T A A K Y K K T V E P L L S L S I D F Y V R V F V K V K T S P I E V K N V M S S T M T T Y H C S R C G S Y H N Q P L G R I S Q R E G R N N K T F T K Y S V A Q G P P V D T K C K F C E G T Y H L A G P M Y A G P L H N K E F I E E V L R I N K E E H R D Q D D T Y G T R K R I E G M L S L A K N E L S D S P F Y F S P N H I A S V I K L Q V P P L K K V V A G L G S L G F E C S L T H A Q P S S L K T N A P W D A I W Y V M Q K C D D E K K D L S K M N P N T T G Y K I L S A M P G W L S G T V K S E Y D S K L S F A P N E Q S G N I E K L R K L K I V R Y Q E N P T K N W G P K A R P N T S

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P15565-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P15565-F1.cif  
DSSP Secondary Structures   P15565.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Isolation and characterization of the TRM1 locus, a gene essential for the N2,N2-dimethylguanosine modification of both mitochondrial and cytoplasmic tRNA in Saccharomyces cerevisiae. Ellis SR, Morales MJ, Li JM, Hopper AK, Martin NC J Biol Chem [details] 2426253 -