Modomics - A Database of RNA Modifications

Full name:
Synonym:	YBR141C
GI:	330443457
Orf:	YBR1118
COG:
UniProt:	P38278
Structures:	\| \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	l:2142(1784) - m1A

Comments:

Intriduces m1A2142 modification of helix 65 in domain IV of 25S rRNA. Predicted to be an Ado-Met dependent methyltransferase from Rossmann fold superfamily. The loss of m1A2142 modification confers anisomycin and peroxide sensitivity to the cells.

Protein sequence:

MHSRKSKSITGKRKQVGSNVTRVIKPQKTRRIIRRFHHLINKRQSICKFLCLKENLDDSNEEKNDKIIRLSIKGNVRLGKYYEDGKSQSFNDAMESQLLR
LHSLIKNESKSKDTSDLAVMYTLLGYIMNQINKLGGLETYQIASQNGQLKERGGDTSKLLEKWIRSSFENCPGAVALEIGSLSSGNRISRCALFRNVVRI
DLEEHEGVIKQDFMERPLPRNENDKFDLISCSLVLNFVKNHRDRGAMCHRMVKFLKPQGYIFIVLPQACVTHSRYCDKTLLQNLLGSIGLIMLNSHQSNK
LYYCLYQLQVVPPQPSSFSKRIKVNDGPGLNNFGITL

Enzymatic activities:

Reaction	Substrate	Type	Position
A:m1A	rRNA (r)	LSU-L/25S/eukaryotic cytosol	2142

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Identification of a novel methyltransferase, Bmt2, responsible for the N-1-methyl-adenosine base modification of 25S rRNA in Saccharomyces cerevisiae	Sunny Sharma, Peter Watzinger, Peter Kötter, and Karl-Dieter Entian	Nucleic Acids Res	[details]	23558746	10.1093/nar/gkt195