Modomics - A Database of RNA Modifications

Full name: tRNA (guanosine(18)-2′-O)-methyltransferase
Synonym:
GI: 74627230
Orf: YDL112W
COG: COG0566
UniProt: Q07527
Structures: | |
Complex:
Enzyme type: methyltransferase
Position of modification - modification: t:18 - Gm

Comments:

AdoMet is the methyl group donor. Site-specific. SPOUT superfamily. Methylates Gm18 in all intron-less yeast tRNAs known to possess this modification.

Protein sequence:

MVGGALICKYLPREEQLKLISDLIQNDSLEEVLELIETSPLDITTDSNIETPIFEKITEQVIAYASIDGEAREMFRSSRAEMNKALRTSAQLLCCLPSVW
HKFQVWMSYRLNDIISENYKHLFNDNFGKKIVQPFFDSFAEEQNANIKHENLHLDILSLLHYLEVVYLFDECKNGISSKCLDFIIVPLLGCNSEEIADSC
SKLMRWHIKYLSKCCNTDSNFDKLIWTFIKQLYAEGSQQAWKQKNSLSFLLRFLLAAELSPELITYIKTDAYWRHIQTELDNDVHEHRKLALSILKLTIQ
KLSSHGITLQTTFYKCNDLANIEMLGSWKKFTTLYEMIALDTSLNQIQAAKQDIIKIFDNEHLHHSWGLILLSTGLKSSMESVRKYMMTLMFSITNMSAF
SSNLPLLTKTLLPAAMSAHYFDVKGVSCPHGEKLSLFVNNLLSQTTEGISDILFEILKLLVEKGTSFDPSRIYLSYGILVFFQNNKQKTINSDHLSLIRK
LYDFAAEEEVLETTIQTIYLKFLLYIDPSVSASELLFTLVSHIKLKGGTYKYVEPLFEDYRDLAVSHFDDLQAKENLTTNIGKDTIFDLLASIIFDFKDI
DITPDFLIEVAKSKQDIPVYTSKAVTFLTQLLSGEPSNGYTYENATALLSYPNFTISTWKSINVNNLFKSVMEKFSLDKFKFFAEIYQKTYECRFDTIEL
NFNDLLSLYEMVKKSANQCSRESFKVKDSAYSSYFELLNTFLKTYALNRDSSEGNDDELHILLRLVDENINKDNGNYLGNLAVCKLLYFIIDSYIHCSTS
VSDDDIFIVKFIFEKFSFIWECINSERLVLKERDLHLMLIKGLFHPVILYFGSNQYIDTLTSKLEEHAQTIISLSYSRRSLLPLLGSQLRVFMKFYGKLL
REDVNYWWLINIIVGVFKQPQMDVNLYKLKPVISSLFDHKLNNYYIKGDELYEKVYGPDEILARVSIIDSILYANDQLKIRLIEKVTEKTNALYAIKRTD
GAEALQRLLQWQLLLLSLLTTNEKKLSETSMIRILKSIEDESSPLVRVYKEWFISSKVVDYYKTGNPKFAEDYLFSLLEDHSKPVFVVSAEKICFMVLKD
LRNDEKKYGFTQLLDRFICTLVPNAASNKPLVRHFSNSLIISLWPTFKAYLSDHTLRNIIENLYSNAKKTQIFGQYRAGDANIWDLKGDRKLTNMFGGVL
KKVTDHDCPYISESVFEKYLQEKDIVPIGTDERSLWLDKRDTNTESVNNANISCDTSPLQTKSGAWETVLDLDNKKSNDVVTRSELIVVSSLVDKPPNLG
GICRLCDVLGVGLLTVQDIKVKNHPQFKNVAVTADRWMPMEEVALDEIASFMKEKKKEGYTLIGLEQTDKSVKLDNNFQFPKKSLILLGTEAFGIPGTLL
SELDLCLEIQQFGVIRSMNIQTATAVIVHSYTVQHM

Enzymatic activities:

Reaction Substrate Type Position
G:Gm tRNA (t) Leu/UAG/prokaryotic cytosol 18
G:Gm tRNA (t) Leu/CAA/prokaryotic cytosol 18
G:Gm tRNA (t) Leu/UAA/prokaryotic cytosol 18
G:Gm tRNA (t) His/GUG/prokaryotic cytosol 18
G:Gm tRNA (t) Tyr/GUA/prokaryotic cytosol 18
G:Gm tRNA (t) Ser/CGA/prokaryotic cytosol 18
G:Gm tRNA (t) Ser/UGA/prokaryotic cytosol 18
G:Gm tRNA (t) Ser/IGA/prokaryotic cytosol 18
G:Gm tRNA (t) Trp/CCA/prokaryotic cytosol 18

Publications:

Title Authors Journal Details PubMed Id DOI
The yeast Saccharomyces cerevisiae YDL112w ORF encodes the putative 2'-O-ribose methyltransferase catalyzing the formation of Gm18 in tRNAs. Cavaille J, Chetouani F, Bachellerie JP RNA [details] 9917067 -

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