Modomics - A Database of RNA Modifications

ID Card:

Full name: 25S rRNA (uridine(2634)-N(3))-methyltransferase
GI: 6322095
Orf: YIL096C
UniProt: P40493
Alpha Fold Predicted Structure: AF-P40493-F1
Enzyme type: methyltransferase
Position of modification - modification: l:2634(2265) - m3U



Protein sequence:

MARKLKGKIGSKGLKGALLRHKAKVKLVRNIESKQKHELRKKNSSANNKTVKRNQEFQKLNQGKVMPFEKDETLMLCGEGDFSFARSIVEQNYIESDNLIITSYDNSVNELKLKYPHTFEENYQYLKDLNIPIFFQIDVTKLVKSFKISKNNTWFKIINRLSDHRWGNKPLQNIVFNFPHNGKGIKDQERNIREHQDLIFNFFQNSLQLFNLINTKIQNDTLRYTQGYDLNEDTPQAKKLTAEGYGNIILSLFDGEPYDSWQIKLLAKKNGLTLSRSSKFQWENFPGYHHRRTNSEQDTTKPAKERDARFYIFSKYVSNSSKHNRKSKKDTDSDSD

Comments:

Btm5 belongs to the Rossmann-fold-like methyltransferases. Point mutations in the S-adenosyl-L-methionine binding pocket abolish the methylation reaction. Bmt5 localizes in the nucleolus. Bmt5 methylates the N3 position of uridine 2634(m3U2634) in the 25s rRNA (Sharma et al. 2013 ).




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
U:m3U RNA rRNA 2634 LSU-25S Nucleolus 24335083   

Alpha Fold Predicted Structure:






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Protein sequence:

M A R K L K G K I G S K G L K G A L L R H K A K V K L V R N I E S K Q K H E L R K K N S S A N N K T V K R N Q E F Q K L N Q G K V M P F E K D E T L M L C G E G D F S F A R S I V E Q N Y I E S D N L I I T S Y D N S V N E L K L K Y P H T F E E N Y Q Y L K D L N I P I F F Q I D V T K L V K S F K I S K N N T W F K I I N R L S D H R W G N K P L Q N I V F N F P H N G K G I K D Q E R N I R E H Q D L I F N F F Q N S L Q L F N L I N T K I Q N D T L R Y T Q G Y D L N E D T P Q A K K L T A E G Y G N I I L S L F D G E P Y D S W Q I K L L A K K N G L T L S R S S K F Q W E N F P G Y H H R R T N S E Q D T T K P A K E R D A R F Y I F S K Y V S N S S K H N R K S K K D T D S D S D

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P40493-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P40493-F1.cif  
DSSP Secondary Structures   P40493.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Identification of novel methyltransferases, Bmt5 and Bmt6, responsible for the m3U methylations of 25S rRNA in Saccharomyces cerevisiae. Sharma S, Yang J, Duttmann S, Watzinger P, Kotter P, Entian KD... Nucleic Acids Res [details] 24335083 -

Links:

_PubMed_