Modomics - A Database of RNA Modifications

ID Card:

Full name: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
Synonym: Tyw1
GI: 14520684
Orf: PAB2039
COG: COG0731
UniProt: Q9V1F9
Alpha Fold Predicted Structure: AF-Q9V1F9-F1
Enzyme type: oxidoreductase
Position of modification - modification: t:37 - mimG



Protein sequence:

MREMITIKPGKITVQANPNMPEEVANLFRKQHYEIVGRHSGVKLCHWLKKSLTEGRFCYKQKFYGIHSHRCLQMTPVLAWCTHNCIFCWRPMETFLGTELPQPWDDPEFIVEESIKAQRKLLIGYKGNPKVDKKKFEEAWEPKHAAISLSGEPMLYPYMGDLVEEFHKRGFTTFIVTNGTVPERLEEMIKEDKLPTQLYVSITAPDIETYNSVNIPMIPDGWERIMRFLELMRDLPTRTVVRLTLVKGENMHSPEKYAKLILKARPMFVEAKAYMFVGYSRNRLTINNMPSHQDIREFAEALVKHLPGYHIEDEYEPSRVVLIMRDDVDPQGTGVNGRFIKH

Comments:

Homolog of yeast Tyw1. Does not have the N-terminal FMN binding/flavodoxin domain found in Tyw1.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
m1G:imG-14 tRNA (t) Phe/GAA/prokaryotic cytosol 37 23043105   

Alpha Fold Predicted Structure:






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Protein sequence:

M R E M I T I K P G K I T V Q A N P N M P E E V A N L F R K Q H Y E I V G R H S G V K L C H W L K K S L T E G R F C Y K Q K F Y G I H S H R C L Q M T P V L A W C T H N C I F C W R P M E T F L G T E L P Q P W D D P E F I V E E S I K A Q R K L L I G Y K G N P K V D K K K F E E A W E P K H A A I S L S G E P M L Y P Y M G D L V E E F H K R G F T T F I V T N G T V P E R L E E M I K E D K L P T Q L Y V S I T A P D I E T Y N S V N I P M I P D G W E R I M R F L E L M R D L P T R T V V R L T L V K G E N M H S P E K Y A K L I L K A R P M F V E A K A Y M F V G Y S R N R L T I N N M P S H Q D I R E F A E A L V K H L P G Y H I E D E Y E P S R V V L I M R D D V D P Q G T G V N G R F I K H

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q9V1F9-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q9V1F9-F1.cif  
DSSP Secondary Structures   Q9V1F9.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
4-Demethylwyosine synthase from Pyrococcus abyssi is a radical-S-adenosyl-L-methionine enzyme with an additional [4Fe-4S](+2) cluster that interacts with the pyruvate co-substrate. Perche-Letuvee P, Kathirvelu V, Berggren G, Clemancey M, Latour JM, Maurel V, Douki T, Armengaud J, Mulliez E, Fontecave M, Garcia-Serres R, Gambarelli S, Atta M... J Biol Chem [details] 23043105 -