ID Card:
Full name:
tRNA (cytosine(49)-C(5))-methyltransferase
Synonym:
PYRAB06230
GI:
14520840
Orf:
PAB1947
COG:
COG0144
UniProt:
Q9V106
Alpha Fold Predicted Structure:
AF-Q9V106-F1
Enzyme type:
methyltransferase
Position of modification - modification:
t :49 - m5C
Protein sequence:
MKRWEHYEHTADIGIRGYGDSLEEAFEAVAIALFDVIVNVNKVEKKEVREVEVEGEDLESLLYNFLEELLVIHDIEGLVFRDFEVKIEKTEKGYKLKAKAYGEKLDPEKHEPKEEVKAITYHDMKIEKLPDGRWMAQLVPDI
Comments:
Catalyzes m5C formation at several cytosines within tRNAs, with preference for C49. The specificity of the enzyme is increased by complex formation with protein archease (PAB1946) encoded upstream from the MTase gene (PAB1947).
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
C:m5C
tRNA (t)
49
17470432
Alpha Fold Predicted Structure:
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Protein sequence:
M
K
R
W
E
H
Y
E
H
T
A
D
I
G
I
R
G
Y
G
D
S
L
E
E
A
F
E
A
V
A
I
A
L
F
D
V
I
V
N
V
N
K
V
E
K
K
E
V
R
E
V
E
V
E
G
E
D
L
E
S
L
L
Y
N
F
L
E
E
L
L
V
I
H
D
I
E
G
L
V
F
R
D
F
E
V
K
I
E
K
T
E
K
G
Y
K
L
K
A
K
A
Y
G
E
K
L
D
P
E
K
H
E
P
K
E
E
V
K
A
I
T
Y
H
D
M
K
I
E
K
L
P
D
G
R
W
M
A
Q
L
V
P
D
I
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
Archease from Pyrococcus abyssi improves substrate specificity and solubility of a tRNA m5C methyltransferase.
Auxilien S, El Khadali F, Rasmussen A, Douthwaite S, Grosjean H...
J Biol Chem
[details]
17470432
-
Links: