Modomics - A Database of RNA Modifications

Full name:	tRNA (cytosine(38)-C(5))-methyltransferase
Synonym:	GSU0227
GI:	39995337
Orf:
COG:
UniProt:	Q74GL9
Structures:	\| \|
Complex:
Enzyme type:	methyltransferase
Position of modification - modification:	t:38 - m5C

Comments:

In contrast to Dnmt2 methyltransferases from eukaryotes which methylate C38 of tRNAAsp, this enzyme methylates C38 in tRNAGlu. This swapped tRNA specificity is dependent on the differences in the sequence of variable loop in the substrate tRNAs.

Protein sequence:

MRAVELFCGIGGFAAAVEGTGVRVVAAMDQDEAALATYRLNFPGHGARKVDLERVSAWELTAGGVDLWWLSPPCQPYCERGVRRDLADPRARSLVHILNL
AARMSDEALPRHLALENVAGFVGSEAHGRLTEVLSSRGYRLQERLLCPTELGIPSRRPRYYLAASRESLAPAEVLSPLPRQPLAEYLDLLPANGQPAELL
LSPAIVERFGAGFRILDPADPDAYTTCFTSGYGRSLTASGAYLRCSDGVRRFSPEEIARLLHFPPSFRFPEEVPLRKRWQLVGNSLSVAAVREVLRALPL
PAEEG

Enzymatic activities:

Reaction	Substrate	Type	Position
C:m5C	tRNA (t)	Glu/unknown/prokaryotic cytosol	38

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
The Dnmt2 RNA methyltransferase homolog of Geobacter sulfurreducens specifically methylates tRNA-Glu.	Shanmugam R, Aklujkar M, Schafer M, Reinhardt R, Nickel O, Reuter G, Lovley DR, Ehrenhofer-Murray A, Nellen W, Ankri S, Helm M, Jurkowski TP, Jeltsch A...	Nucleic Acids Res	[details]	24711368	-

Links:

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