ID Card:
Full name:
Ribosomal RNA cytidine Acetyltransferase 1
Synonym:
KRE33
GI:
6324197
Orf:
N1216, N1858
COG:
COG1444
UniProt:
P53914
Structures:
|
6KE6 |
6LQP |
6LQQ |
6LQR |
6LQU |
6LQV |
6ZQB |
6ZQC |
7AJT |
7D63 |
7SUK |
Alpha Fold Predicted Structure:
AF-P53914-F1
Enzyme type:
acetyltransferase
Position of modification - modification:
t :12 - ac4C s :1773(1510) - ac4C s :1280(1060) - ac4C
PDB Structures:
6KE6
6LQP
6LQQ
6LQR
6LQU
6LQV
6ZQB
6ZQC
7AJT
7D63
7SUK
}
6KE6
Structure Description:
Abstract of the PDB Structure's related Publication:
Download RCSB-PDB Structures:
Protein sequence:
MAKKAIDSRIPSLIRNGVQTKQRSIFVIVGDRARNQLPNLHYLMMSADLKMNKSVLWAYKKKLLGFTSHRKKRENKIKKEIKRGTREVNEMDPFESFISNQNIRYVYYKESEKILGNTYGMCILQDFEALTPNLLARTIETVEGGGIVVILLKSMSSLKQLYTMTMDVHARYRTEAHGDVVARFNERFILSLGSNPNCLVVDDELNVLPLSGAKNVKPLPPKEDDELPPKQLELQELKESLEDVQPAGSLVSLSKTVNQAHAILSFIDAISEKTLNFTVALTAGRGRGKSAALGISIAAAVSHGYSNIFVTSPSPENLKTLFEFIFKGFDALGYQEHIDYDIIQSTNPDFNKAIVRVDIKRDHRQTIQYIVPQDHQVLGQAELVVIDEAAAIPLPIVKNLLGPYLVFMASTINGYEGTGRSLSLKLIQQLRNQNNTSGRESTQTAVVSRDNKEKDSHLHSQSRQLREISLDEPIRYAPGDPIEKWLNKLLCLDVTLIKNPRFATRGTPHPSQCNLFVVNRDTLFSYHPVSENFLEKMMALYVSSHYKNSPNDLQLMSDAPAHKLFVLLPPIDPKDGGRIPDPLCVIQIALEGEISKESVRNSLSRGQRAGGDLIPWLISQQFQDEEFASLSGARIVRIATNPEYASMGYGSRAIELLRDYFEGKFTDMSEDVRPKDYSIKRVSDKELAKTNLLKDDVKLRDAKTLPPLLLKLSEQPPHYLHYLGVSYGLTQSLHKFWKNNSFVPVYLRQTANDLTGEHTCVMLNVLEGRESNWLVEFAKDFRKRFLSLLSYDFHKFTAVQALSVIESSKKAQDLSDDEKHDNKELTRTHLDDIFSPFDLKRLDSYSNNLLDYHVIGDMIPMLALLYFGDKMGDSVKLSSVQSAILLAIGLQRKNIDTIAKELNLPSNQTIAMFAKIMRKMSQYFRQLLSQSIEETLPNIKDDAIAEMDGEEIKNYNAAEALDQMEEDLEEAGSEAVQAMREKQKELINSLNLDKYAINDNSEEWAESQKSLEIAAKAKGVVSLKTGKKRTTEKAEDIYRQEMKAMKKPRKSKKAAN
Comments:
RRA1 (KRE33) is a cytosine acetyltransferase with specificity towards both 18S rRNA and serine and leucine tRNAs. KRE33 is required for formation of ac4C1280 (helix 34) and ac4C1773 (helix 45) on 18S rRNA. KRE33 is required for pre-rRNA processing steps leading to 18S rRNA synthesis but 18S rRNA acetylation is not required for pre-rRNA processing. KRE33 is additionally required for ac4C12 on serine and leucine tRNAs. KRE33 interacts in vivo with TAN1. TAN1 is also required for tRNA acetylation but does not carry any acetyltransferase domain. On this basis, TAN1 was suggested to act as a tRNA adaptor to KRE33. NAT10 is the human homolog of KRE33 and THUMPD1 the human homolog of TAN1.
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
C:ac4C
rRNA (r)
SSU/18S/eukaryotic cytosol
1773
25086048
C:ac4C
rRNA (r)
SSU/18S/eukaryotic cytosol
1280
25653167
C:ac4C
tRNA (t)
Leu/UAG/eukaryotic cytosol
12
25653167
C:ac4C
tRNA (t)
Leu/CAA/eukaryotic cytosol
12
25653167
C:ac4C
tRNA (t)
Leu/UAA/eukaryotic cytosol
12
25653167
C:ac4C
tRNA (t)
Ser/CGA/eukaryotic cytosol
12
25653167
C:ac4C
tRNA (t)
Ser/UGA/eukaryotic cytosol
12
25653167
C:ac4C
tRNA (t)
Ser/IGA/eukaryotic cytosol
12
25653167
C:ac4C
tRNA (t)
Ser/GCU/eukaryotic cytosol
12
25653167
Alpha Fold Predicted Structure:
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Protein sequence:
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K
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Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
A single acetylation of 18S rRNA is essential for biogenesis of the small ribosomal subunit in Saccharomyces cerevisiae.
Ito S, Akamatsu Y, Noma A, Kimura S, Miyauchi K, Ikeuchi Y, Suzuki T, Suzuki T...
J Biol Chem
[details]
25086048
-
Yeast Kre33 and human NAT10 are conserved 18S rRNA cytosine acetyltransferases that modify tRNAs assisted by the adaptor Tan1/THUMPD1.
Sharma S, Langhendries JL, Watzinger P, Kotter P, Entian KD, Lafontaine DL...
Nucleic Acids Res
[details]
25653167
-
Links: