| Full name: |
Ribosomal RNA cytidine Acetyltransferase 1 |
| Synonym: |
KRE33 |
| GI: |
6324197 |
| Orf: |
N1216, N1858 |
| COG: |
COG1444 |
| UniProt: |
P53914 |
| Structures: |
|
|
|
| Complex: |
|
| Enzyme type: |
acetyltransferase |
| Position of modification - modification: |
t:12 - ac4C
s:1773(1510) - ac4C
s:1280(1060) - ac4C
|
Comments:
RRA1 (KRE33) is a cytosine acetyltransferase with specificity towards both 18S rRNA and serine and leucine tRNAs. KRE33 is required for formation of ac4C1280 (helix 34) and ac4C1773 (helix 45) on 18S rRNA. KRE33 is required for pre-rRNA processing steps leading to 18S rRNA synthesis but 18S rRNA acetylation is not required for pre-rRNA processing. KRE33 is additionally required for ac4C12 on serine and leucine tRNAs. KRE33 interacts in vivo with TAN1. TAN1 is also required for tRNA acetylation but does not carry any acetyltransferase domain. On this basis, TAN1 was suggested to act as a tRNA adaptor to KRE33. NAT10 is the human homolog of KRE33 and THUMPD1 the human homolog of TAN1.
Protein sequence:
MAKKAIDSRIPSLIRNGVQTKQRSIFVIVGDRARNQLPNLHYLMMSADLKMNKSVLWAYKKKLLGFTSHR
KKRENKIKKEIKRGTREVNEMDPFESFISNQNIRYVYYKESEKILGNTYGMCILQDFEALTPNLLARTIE
TVEGGGIVVILLKSMSSLKQLYTMTMDVHARYRTEAHGDVVARFNERFILSLGSNPNCLVVDDELNVLPL
SGAKNVKPLPPKEDDELPPKQLELQELKESLEDVQPAGSLVSLSKTVNQAHAILSFIDAISEKTLNFTVA
LTAGRGRGKSAALGISIAAAVSHGYSNIFVTSPSPENLKTLFEFIFKGFDALGYQEHIDYDIIQSTNPDF
NKAIVRVDIKRDHRQTIQYIVPQDHQVLGQAELVVIDEAAAIPLPIVKNLLGPYLVFMASTINGYEGTGR
SLSLKLIQQLRNQNNTSGRESTQTAVVSRDNKEKDSHLHSQSRQLREISLDEPIRYAPGDPIEKWLNKLL
CLDVTLIKNPRFATRGTPHPSQCNLFVVNRDTLFSYHPVSENFLEKMMALYVSSHYKNSPNDLQLMSDAP
AHKLFVLLPPIDPKDGGRIPDPLCVIQIALEGEISKESVRNSLSRGQRAGGDLIPWLISQQFQDEEFASL
SGARIVRIATNPEYASMGYGSRAIELLRDYFEGKFTDMSEDVRPKDYSIKRVSDKELAKTNLLKDDVKLR
DAKTLPPLLLKLSEQPPHYLHYLGVSYGLTQSLHKFWKNNSFVPVYLRQTANDLTGEHTCVMLNVLEGRE
SNWLVEFAKDFRKRFLSLLSYDFHKFTAVQALSVIESSKKAQDLSDDEKHDNKELTRTHLDDIFSPFDLK
RLDSYSNNLLDYHVIGDMIPMLALLYFGDKMGDSVKLSSVQSAILLAIGLQRKNIDTIAKELNLPSNQTI
AMFAKIMRKMSQYFRQLLSQSIEETLPNIKDDAIAEMDGEEIKNYNAAEALDQMEEDLEEAGSEAVQAMR
EKQKELINSLNLDKYAINDNSEEWAESQKSLEIAAKAKGVVSLKTGKKRTTEKAEDIYRQEMKAMKKPRK
SKKAAN
Enzymatic activities:
| Reaction
| Substrate
| Type
| Position
|
|
C:ac4C
|
rRNA (r) |
SSU/18S/cytosolic |
1773 |
|
C:ac4C
|
rRNA (r) |
SSU/18S/cytosolic |
1280 |
|
C:ac4C
|
tRNA (t) |
Leu/UAG/cytosolic |
12 |
|
C:ac4C
|
tRNA (t) |
Leu/CAA/cytosolic |
12 |
|
C:ac4C
|
tRNA (t) |
Leu/UAA/cytosolic |
12 |
|
C:ac4C
|
tRNA (t) |
Ser/CGA/cytosolic |
12 |
|
C:ac4C
|
tRNA (t) |
Ser/UGA/cytosolic |
12 |
|
C:ac4C
|
tRNA (t) |
Ser/IGA/cytosolic |
12 |
|
C:ac4C
|
tRNA (t) |
Ser/GCU/cytosolic |
12 |
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| A single acetylation of 18S rRNA is essential for biogenesis of the small ribosomal subunit in Saccharomyces cerevisiae. |
Ito S, Akamatsu Y, Noma A, Kimura S, Miyauchi K, Ikeuchi Y, Suzuki T, Suzuki T... |
J Biol Chem |
[details]
|
25086048
|
-
|
| Yeast Kre33 and human NAT10 are conserved 18S rRNA cytosine acetyltransferases that modify tRNAs assisted by the adaptor Tan1/THUMPD1. |
Sharma S, Langhendries JL, Watzinger P, Kotter P, Entian KD, Lafontaine DL... |
Nucleic Acids Res |
[details]
|
25653167
|
-
|
Links: