ID Card:
Full name:
tRNA-methyltransferase O
Synonym:
TsaA, YaeB
GI:
16128188
COG:
COG1720
UniProt:
P28634
Alpha Fold Predicted Structure:
AF-P28634-F1
Enzyme type:
methyltransferase
Position of modification - modification:
t :37 - m6t6A
Protein sequence:
MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGMSLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAEMAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNVRWRVTDAGFEVFALEPR
Comments:
TrmO has a unique single-sheeted beta-barrel structure and does not belong to any known classes of methyltransferases (as for August 2014). It employs S-adenosyl-L-methionine as a methyl donor and hence represents a novel category of AdoMet-dependent methyltransferase (Class VIII). In the tRNA substrate C31-G39 and G34 are major determinants of m6t6A formation.
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
t6A:m6t6A
tRNA (t)
Thr/GGU/prokaryotic cytosol
37
25063302
Alpha Fold Predicted Structure:
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Protein sequence:
M
S
S
F
Q
F
E
Q
I
G
V
I
R
S
P
Y
K
E
K
F
A
V
P
R
Q
P
G
L
V
K
S
A
N
G
E
L
H
L
I
A
P
Y
N
Q
A
D
A
V
R
G
L
E
A
F
S
H
L
W
I
L
F
V
F
H
Q
T
M
E
G
G
W
R
P
T
V
R
P
P
R
L
G
G
N
A
R
M
G
V
F
A
T
R
S
T
F
R
P
N
P
I
G
M
S
L
V
E
L
K
E
V
V
C
H
K
D
S
V
I
L
K
L
G
S
L
D
L
V
D
G
T
P
V
V
D
I
K
P
Y
L
P
F
A
E
S
L
P
D
A
S
A
S
Y
A
Q
S
A
P
A
A
E
M
A
V
S
F
T
A
E
V
E
K
Q
L
L
T
L
E
K
R
Y
P
Q
L
T
L
F
I
R
E
V
L
A
Q
D
P
R
P
A
Y
R
K
G
E
E
T
G
K
T
Y
A
V
W
L
H
D
F
N
V
R
W
R
V
T
D
A
G
F
E
V
F
A
L
E
P
R
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
The methyl group of the N6-methyl-N6-threonylcarbamoyladenosine in tRNA of Escherichia coli modestly improves the efficiency of the tRNA.
Qian Q, Curran JF, Bjork GR
J Bacteriol
[details]
9537379
-
Discovery of the beta-barrel-type RNA methyltransferase responsible for N6-methylation of N6-threonylcarbamoyladenosine in tRNAs.
Kimura S, Miyauchi K, Ikeuchi Y, Thiaville PC, de Crecy-Lagard V, Suzuki T...
Nucleic Acids Res
[details]
25063302
-
Links: