Modomics - A Database of RNA Modifications

ID Card:

Full name: tRNA-methyltransferase O
Synonym: TsaA, YaeB
GI: 16128188
COG: COG1720
UniProt: P28634
Alpha Fold Predicted Structure: AF-P28634-F1
Enzyme type: methyltransferase
Position of modification - modification: t:37 - m6t6A



Protein sequence:

MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGMSLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAEMAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNVRWRVTDAGFEVFALEPR

Comments:

TrmO has a unique single-sheeted beta-barrel structure and does not belong to any known classes of methyltransferases (as for August 2014). It employs S-adenosyl-L-methionine as a methyl donor and hence represents a novel category of AdoMet-dependent methyltransferase (Class VIII). In the tRNA substrate C31-G39 and G34 are major determinants of m6t6A formation.





Alpha Fold Predicted Structure:




Parsing response... [221979/221979]


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Protein sequence:

M S S F Q F E Q I G V I R S P Y K E K F A V P R Q P G L V K S A N G E L H L I A P Y N Q A D A V R G L E A F S H L W I L F V F H Q T M E G G W R P T V R P P R L G G N A R M G V F A T R S T F R P N P I G M S L V E L K E V V C H K D S V I L K L G S L D L V D G T P V V D I K P Y L P F A E S L P D A S A S Y A Q S A P A A E M A V S F T A E V E K Q L L T L E K R Y P Q L T L F I R E V L A Q D P R P A Y R K G E E T G K T Y A V W L H D F N V R W R V T D A G F E V F A L E P R
20406080100120140160180200220SequenceGHTBSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-P28634-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-P28634-F1.cif  
DSSP Secondary Structures   P28634.dssp  





Publications:

Links:

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