ID Card:
Full name:
tRNA-methyltransferase O
GI:
118600979
Orf:
HSPC219
COG:
COG1720
UniProt:
Q9BU70
Alpha Fold Predicted Structure:
AF-Q9BU70-F1
Enzyme type:
methyltransferase
Position of modification - modification:
t : - m6t6A
Protein sequence:
MRGLEESGPRPTATPCGCVKPALETGNLLTEPVGYLESCFSAKNGTPRQPSICSYSRACLRIRKRIFNNPEHSLMGLEQFSHVWILFVFHKNGHLSCKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAIYLSGIDMIHGTPVLDIKPYIAEYDSPQNVMEPLADFNLQNNQHTPNTVSQSDSKTDSCDQRQLSGCDEPQPHHSTKRKPKCPEDRTSEENYLTHSDTARIQQAFPMHREIAVDFGLESRRDQSSSVAEEQIGPYCPEKSFSEKGTDKKLERVEGAAVLQGSRAETQPMAPHCPAGRADGAPRSVVPAWVTEAPVATLEVRFTPHAEMDLGQLSSQDVGQASFKYFQSAEEAKRAIEAVLSADPRSVYRRKLCQDRLFYFTVDIAHVTCWFGDGFAEVLRIKPASEPVHMTGPVGSLVSLGS
Comments:
Homolog of E. coli TrmO. The substrate recognition mechanism of human TRMO differs from that of E. coli TrmO - human tRNASer(GCU) has A31-U39 and C35, which should function as anti-determinants for E. coli TrmO.
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
t6A:m6t6A
tRNA (t)
Ser/GCU/eukaryotic cytosol
37
25063302
Alpha Fold Predicted Structure:
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Protein sequence:
M
R
G
L
E
E
S
G
P
R
P
T
A
T
P
C
G
C
V
K
P
A
L
E
T
G
N
L
L
T
E
P
V
G
Y
L
E
S
C
F
S
A
K
N
G
T
P
R
Q
P
S
I
C
S
Y
S
R
A
C
L
R
I
R
K
R
I
F
N
N
P
E
H
S
L
M
G
L
E
Q
F
S
H
V
W
I
L
F
V
F
H
K
N
G
H
L
S
C
K
A
K
V
Q
P
P
R
L
N
G
A
K
T
G
V
F
S
T
R
S
P
H
R
P
N
A
I
G
L
T
L
A
K
L
E
K
V
E
G
G
A
I
Y
L
S
G
I
D
M
I
H
G
T
P
V
L
D
I
K
P
Y
I
A
E
Y
D
S
P
Q
N
V
M
E
P
L
A
D
F
N
L
Q
N
N
Q
H
T
P
N
T
V
S
Q
S
D
S
K
T
D
S
C
D
Q
R
Q
L
S
G
C
D
E
P
Q
P
H
H
S
T
K
R
K
P
K
C
P
E
D
R
T
S
E
E
N
Y
L
T
H
S
D
T
A
R
I
Q
Q
A
F
P
M
H
R
E
I
A
V
D
F
G
L
E
S
R
R
D
Q
S
S
S
V
A
E
E
Q
I
G
P
Y
C
P
E
K
S
F
S
E
K
G
T
D
K
K
L
E
R
V
E
G
A
A
V
L
Q
G
S
R
A
E
T
Q
P
M
A
P
H
C
P
A
G
R
A
D
G
A
P
R
S
V
V
P
A
W
V
T
E
A
P
V
A
T
L
E
V
R
F
T
P
H
A
E
M
D
L
G
Q
L
S
S
Q
D
V
G
Q
A
S
F
K
Y
F
Q
S
A
E
E
A
K
R
A
I
E
A
V
L
S
A
D
P
R
S
V
Y
R
R
K
L
C
Q
D
R
L
F
Y
F
T
V
D
I
A
H
V
T
C
W
F
G
D
G
F
A
E
V
L
R
I
K
P
A
S
E
P
V
H
M
T
G
P
V
G
S
L
V
S
L
G
S
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
Discovery of the beta-barrel-type RNA methyltransferase responsible for N6-methylation of N6-threonylcarbamoyladenosine in tRNAs.
Kimura S, Miyauchi K, Ikeuchi Y, Thiaville PC, de Crecy-Lagard V, Suzuki T...
Nucleic Acids Res
[details]
25063302
-