| Full name: |
N-acetyltransferase 10 |
| Synonym: |
ALP, KIAA1709 |
| GI: |
221316723 |
| Orf: |
|
| COG: |
COG1444 |
| UniProt: |
Q9H0A0 |
| Structures: |
|
|
|
| Complex: |
|
| Enzyme type: |
acetyltransferase |
| Position of modification - modification: |
s:1338(1060) - ac4C
s:1843(1510) - ac4C
|
Comments:
NAT10 is the human homolog of yeast RRA1. It is a disease-associated gene (progeria, laminopathies). NAT10 is required for the formation of two ac4C in human SSU rRNA: one in helix 34, predicted, based on the yeast work, to map to position 1338, and one in helix 45, predicted to be at position 1843. siRNA-mediated depletion of NAT10 also leads to loss of tRNA acetylation. NAT10 interacts in vivo in human cells with THUMPD1, the homolog of TAN1, and a NAT10/THUMPD1 complex is suggested to work in tRNA acetylation in human, similar to the KRE33/TAN1 complex in budding yeast. NAT10 complements both 18S rRNA and tRNA acetylation in yeast.
Protein sequence:
MHRKKVDNRIRILIENGVAERQRSLFVVVGDRGKDQVVILHHMLSKATVKARPSVLWCYKKELGFSSHRK
KRMRQLQKKIKNGTLNIKQDDPFELFIAATNIRYCYYNETHKILGNTFGMCVLQDFEALTPNLLARTVET
VEGGGLVVILLRTMNSLKQLYTVTMDVHSRYRTEAHQDVVGRFNERFILSLASCKKCLVIDDQLNILPIS
SHVATMEALPPQTPDESLGPSDLELRELKESLQDTQPVGVLVDCCKTLDQAKAVLKFIEGISEKTLRSTV
ALTAARGRGKSAALGLAIAGAVAFGYSNIFVTSPSPDNLHTLFEFVFKGFDALQYQEHLDYEIIQSLNPE
FNKAVIRVNVFREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKSLLGPYLVFMASTINGYEGTG
RSLSLKLIQQLRQQSAQSQVSTTAENKTTTTARLASARTLHEVSLQESIRYAPGDAVEKWLNDLLCLDCL
NITRIVSGCPLPEACELYYVNRDTLFCYHKASEVFLQRLMALYVASHYKNSPNDLQMLSDAPAHHLFCLL
PPVPPTQNALPEVLAVIQVCLEGEISRQSILNSLSRGKKASGDLIPWTVSEQFQDPDFGGLSGGRVVRIA
VHPDYQGMGYGSRALQLLQMYYEGRFPCLEEKVLETPQEIHTVSSEAVSLLEEVITPRKDLPPLLLKLNE
RPAERLDYLGVSYGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKD
FRRRFLALLSYQFSTFSPSLALNIIQNRNMGKPAQPALSREELEALFLPYDLKRLEMYSRNMVDYHLIMD
MIPAISRIYFLNQLGDLALSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIRKVVKLFNEVQ
EKAIEEQMVAAKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKSMDLSEYIIRGDDEEWNEVLNKAG
PNASIISLKSDKKRKLEAKQEPKQSKKLKNRETKNKKDMKLKRKK
Enzymatic activities:
| Reaction
| Substrate
| Type
| Position
|
|
C:ac4C
|
rRNA (r) |
SSU/18S/cytosolic |
1843 |
|
C:ac4C
|
rRNA (r) |
SSU/18S/cytosolic |
1338 |
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| Human NAT10 is an ATP-dependent RNA acetyltransferase responsible for N4-acetylcytidine formation in 18S rRNA. |
Ito S, Horikawa S, Suzuki T, Kawauchi H, Tanaka Y, Suzuki T, Suzuki T... |
J Biol Chem |
[details]
|
25411247
|
-
|
| Yeast Kre33 and human NAT10 are conserved 18S rRNA cytosine acetyltransferases that modify tRNAs assisted by the adaptor Tan1/THUMPD1. |
Sharma S, Langhendries JL, Watzinger P, Kotter P, Entian KD, Lafontaine DL... |
Nucleic Acids Res |
[details]
|
25653167
|
-
|
Links: