ID Card:
Full name:
Archaeosine tRNA-guanine transglycosylase
Synonym:
queuine tRNA-ribosyltransferase related protein
UniProt:
Q9HI54
Alpha Fold Predicted Structure:
AF-Q9HI54-F1
Enzyme type:
transglycosylase
Position of modification - modification:
t :13 - G+ t :15 - G+
Protein sequence:
MKIEERDGLARIAKFETPHGPIETPTVLPVINPNIMNITPQEMKPLGLQGIITNSYIILRTPELRERALREGLHSLIGYDGPIMTDSGTFQSYVYGSIEFNNREVVDFQRRIGSDISTILDVFTTPGTPKPEAEKAVIETYNRMLEVNDEEGIIAGPVQGGVYPDLRQKSAELMNSTNAGYHPIGGVVPLLETYDYSTLVDIIINSKINLSFNKPVHLFGGGHPMFFAFSVYLGVDLFDSASYIKYAKDDRLIYPDGTRDLARITELPQWSPLYDRYTVKEIRDLDKERRSLEIARHNLKAIFMEISEIKERIYEEGLAQYVAQKARSHPSLMKAYSRIMSYSNILEKYEDLSKKTAYFFYDSFSTRNPYVSRINRFTESYLSSNKKDTYAFTYRAWNPGYTNSEFVRDVYQKIDCNALISWSGTFVPAELENTYPIEQTVSSGFEPDPDFSRAKDLIAPFRVDMYKGEKFEGEQVRSFNLNKIRMVADYQFGSGVGRMIFRDDVRINVSKTGRIRGILSKEGRQIATMRNDGFFTLTYYGASLIHSQLKPPAMRVTVSKESAEYNAKGYSVFFKFILGSDENIIAKNDVLVVDEDDVLAAVGKAMVSGRELREYTEGIAVKVHEGRDQSEK
Comments:
Archaeosine tRNA-guanine transglycosylase (ArcTGT) catalyses the replacement of guanine base with preQ(0) in archaea tRNAs. G(+), the following modification of preQ(0) is catalyzed by archaeosine synthetases. It is found only in position 15 in many archaeal tRNAs. Exceptionally, G(+) is found either in positions 13 and 15 in T.acidophilum , with ArcTGT from T.acidophilum having multisite specificity. ArcTGT is responsible for the formation of both G(+) 13 and G(+) 15 (Kawamura et al. 2016 ).
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
G:preQ0
RNA
tRNA
13
UAG
UAG
tRNALeu UAG
D-stem
prokaryotic cytosol
26721388
G:preQ0
RNA
tRNA
15
UAG
UAG
tRNALeu UAG
D.loop
prokaryotic cytosol
26721388
Alpha Fold Predicted Structure:
Clear Selection and Reset Camera
Clear Selection
Reset Camera
Protein sequence:
M
K
I
E
E
R
D
G
L
A
R
I
A
K
F
E
T
P
H
G
P
I
E
T
P
T
V
L
P
V
I
N
P
N
I
M
N
I
T
P
Q
E
M
K
P
L
G
L
Q
G
I
I
T
N
S
Y
I
I
L
R
T
P
E
L
R
E
R
A
L
R
E
G
L
H
S
L
I
G
Y
D
G
P
I
M
T
D
S
G
T
F
Q
S
Y
V
Y
G
S
I
E
F
N
N
R
E
V
V
D
F
Q
R
R
I
G
S
D
I
S
T
I
L
D
V
F
T
T
P
G
T
P
K
P
E
A
E
K
A
V
I
E
T
Y
N
R
M
L
E
V
N
D
E
E
G
I
I
A
G
P
V
Q
G
G
V
Y
P
D
L
R
Q
K
S
A
E
L
M
N
S
T
N
A
G
Y
H
P
I
G
G
V
V
P
L
L
E
T
Y
D
Y
S
T
L
V
D
I
I
I
N
S
K
I
N
L
S
F
N
K
P
V
H
L
F
G
G
G
H
P
M
F
F
A
F
S
V
Y
L
G
V
D
L
F
D
S
A
S
Y
I
K
Y
A
K
D
D
R
L
I
Y
P
D
G
T
R
D
L
A
R
I
T
E
L
P
Q
W
S
P
L
Y
D
R
Y
T
V
K
E
I
R
D
L
D
K
E
R
R
S
L
E
I
A
R
H
N
L
K
A
I
F
M
E
I
S
E
I
K
E
R
I
Y
E
E
G
L
A
Q
Y
V
A
Q
K
A
R
S
H
P
S
L
M
K
A
Y
S
R
I
M
S
Y
S
N
I
L
E
K
Y
E
D
L
S
K
K
T
A
Y
F
F
Y
D
S
F
S
T
R
N
P
Y
V
S
R
I
N
R
F
T
E
S
Y
L
S
S
N
K
K
D
T
Y
A
F
T
Y
R
A
W
N
P
G
Y
T
N
S
E
F
V
R
D
V
Y
Q
K
I
D
C
N
A
L
I
S
W
S
G
T
F
V
P
A
E
L
E
N
T
Y
P
I
E
Q
T
V
S
S
G
F
E
P
D
P
D
F
S
R
A
K
D
L
I
A
P
F
R
V
D
M
Y
K
G
E
K
F
E
G
E
Q
V
R
S
F
N
L
N
K
I
R
M
V
A
D
Y
Q
F
G
S
G
V
G
R
M
I
F
R
D
D
V
R
I
N
V
S
K
T
G
R
I
R
G
I
L
S
K
E
G
R
Q
I
A
T
M
R
N
D
G
F
F
T
L
T
Y
Y
G
A
S
L
I
H
S
Q
L
K
P
P
A
M
R
V
T
V
S
K
E
S
A
E
Y
N
A
K
G
Y
S
V
F
F
K
F
I
L
G
S
D
E
N
I
I
A
K
N
D
V
L
V
V
D
E
D
D
V
L
A
A
V
G
K
A
M
V
S
G
R
E
L
R
E
Y
T
E
G
I
A
V
K
V
H
E
G
R
D
Q
S
E
K
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
Multisite-specific archaeosine tRNA-guanine transglycosylase (ArcTGT) from Thermoplasma acidophilum, a thermo-acidophilic archaeon.
Kawamura T, Hirata A, Ohno S, Nomura Y, Nagano T, Nameki N, Yokogawa T, Hori H
Nucleic Acids Res.
[details]
26721388
10.1093/nar/gkv1522