Modomics - A Database of RNA Modifications

ID Card:

Full name: cytosolic thiouridylase subunit 1 - Cytoplasmic tRNA 2-thiolation protein 1
Synonym: MGC17332, NCS6
GI: 74713747
UniProt: Q7Z7A3
Alpha Fold Predicted Structure: AF-Q7Z7A3-F1
Enzyme type: thiolase



Protein sequence:

MPAPPCASCHAARAALRRPLSGQALCGACFCAAFEAEVLHTVLAGRLLPPGAVVAVGASGGKDSTVLAHVLRALAPRLGISLQLVAVDEGIGGYRDAALAAVRRQAARWELPLTVVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARRVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGGLGSPGEGGALPRCRPLQFASQKEVVLYAHFRRLDYFSEECVYAPEAFRGHARDLLKRLEAARPSAVLDLVHSAERLALAPAARPPRPGACSRCGALASRALCQACALLDGLNRGRPRLAIGKGRRGLDEEATPGTPGDPARPPASKAVPTF

Comments:

It is speculated how URM1could contribute as a sulfur donor for the thiolation of tRNAGlnUUG, tRNAGluUUC, and tRNALysUUU allowing for the catalysis of tRNA adenylation, an intermediate that is required for 2-thiolation. Deletion of URM1 causes an increased mobility of tRNAGlnUUG, tRNAGluUUC, tRNALysUUU thus indicating the pivotal role of CTU1 in the 2-thiolation of the wobble positions of these three tRNAs (Schlieker et al. 2008 ).




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
U:mcm5s2U RNA tRNA 34 UUG 3UG tRNAGlnUUG anticodon Cytoplasm
U:mcm5s2U RNA tRNA 34 UUC 3UC tRNAGluUUC anticodon Nucleoplasm 19017811   
U:mcm5s2U RNA tRNA 34 UUU 3UU tRNALysUUU wobble position Nucleoplasm 19017811   

Alpha Fold Predicted Structure:






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Protein sequence:

M P A P P C A S C H A A R A A L R R P L S G Q A L C G A C F C A A F E A E V L H T V L A G R L L P P G A V V A V G A S G G K D S T V L A H V L R A L A P R L G I S L Q L V A V D E G I G G Y R D A A L A A V R R Q A A R W E L P L T V V A Y E D L F G G W T M D A V A R S T A G S G R S R S C C T F C G V L R R R A L E E G A R R V G A T H I V T G H N A D D M A E T V L M N F L R G D A G R L A R G G G L G S P G E G G A L P R C R P L Q F A S Q K E V V L Y A H F R R L D Y F S E E C V Y A P E A F R G H A R D L L K R L E A A R P S A V L D L V H S A E R L A L A P A A R P P R P G A C S R C G A L A S R A L C Q A C A L L D G L N R G R P R L A I G K G R R G L D E E A T P G T P G D P A R P P A S K A V P T F

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q7Z7A3-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q7Z7A3-F1.cif  
DSSP Secondary Structures   Q7Z7A3.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
A functional proteomics approach links the ubiquitin-related modifier Urm1 to a tRNA modification pathway. Schlieker CD, Van der Veen AG, Damon JR, Spooner E, Ploegh HL... Proc Natl Acad Sci U S A [details] 19017811 -