Modomics - A Database of RNA Modifications

Full name:	tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61
Synonym:	GCD14
GI:	6322336
Orf:	YJL125C
COG:	COG2519
UniProt:	P46959
Structures:	\| \|
Complex:	Trm6/Trm61
Enzyme type:	methyltransferase
Position of modification - modification:	t:58 - m1A

Comments:

AdoMet is the methyl group donor. Heterotetramer composed of mutually related subunits: 2 copies of Trm6p and two copies of Trm61p. Trm61p is a catalytic subunit, Trm6p is a tRNA-binding subunit. Trm6/Trm61 are structuraly related.

Protein sequence:

MSTNCFSGYKDLIKEGDLTLIWVSRDNIKPVRMHSEEVFNTRYGSFPHKDIIGKPYGSQIAIRTKGSNKFAFVHVLQPTPELWTLSLPHRTQIVYTPDSS
YIMQRLNCSPHSRVIEAGTGSGSFSHAFARSVGHLFSFEFHHIRYEQALEEFKEHGLIDDNVTITHRDVCQGGFLIKKGDTTSYEFGNNETAASLNANVV
FLDLPAPWDAIPHLDSVISVDEKVGLCCFSPCIEQVDKTLDVLEKYGWTDVEMVEIQGRQYESRRQMVRSLNDALERLRDIKRHKLQGVERRKRMFNNTI
DSNDEKVGKRNEDGVPLTEKAKFNPFGKGSRIKEGDSNYKWKEVTKMEAEIKSHTSYLTFAFKVVNRSRDDEKVNEILRSTEK

Enzymatic activities:

Reaction	Substrate	Type	Position
A:m1A	tRNA (t)	Arg/ACU/prokaryotic cytosol	58
A:m1A	tRNA (t)	Arg/ICG/prokaryotic cytosol	58
A:m1A	tRNA (t)	Asn/GUU/prokaryotic cytosol	58
A:m1A	tRNA (t)	Cys/GCA/prokaryotic cytosol	58
A:m1A	tRNA (t)	Ile/IAU/prokaryotic cytosol	58
A:m1A	tRNA (t)	Ile/UAU/prokaryotic cytosol	58
A:m1A	tRNA (t)	Leu/UAG/prokaryotic cytosol	58
A:m1A	tRNA (t)	Leu/UAA/prokaryotic cytosol	58
A:m1A	tRNA (t)	Lys/CUU/prokaryotic cytosol	58
A:m1A	tRNA (t)	Lys/SUU/prokaryotic cytosol	58
A:m1A	tRNA (t)	Met/CAU/prokaryotic cytosol	58
A:m1A	tRNA (t)	Phe/GAA/prokaryotic cytosol	58
A:m1A	tRNA (t)	Pro/UGG/prokaryotic cytosol	58
A:m1A	tRNA (t)	Thr/IGU/prokaryotic cytosol	58
A:m1A	tRNA (t)	Trp/CCA/prokaryotic cytosol	58
A:m1A	tRNA (t)	Tyr/GUA/prokaryotic cytosol	58
A:m1A	tRNA (t)	Val/UAC/prokaryotic cytosol	58
A:m1A	tRNA (t)	Val/CAC/prokaryotic cytosol	58
A:m1A	tRNA (t)	Val/IAC/prokaryotic cytosol	58

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA.	Anderson J, Phan L, Cuesta R, Carlson BA, Pak M, Asano K, Bjork GR, Tamame M, Hinnebusch AG	Genes Dev	[details]	9851972	-
Conserved amino acids in each subunit of the heteroligomeric tRNA m1A58 Mtase from Saccharomyces cerevisiae contribute to tRNA binding.	Ozanick SG, Bujnicki JM, Sem DS, Anderson JT	Nucleic Acids Res	[details]	17932071	-