Modomics - A Database of RNA Modifications

Full name:	tRNA 2-selenouridine(34) synthase
Synonym:	SelU, YbbB
GI:	2506609
Orf:	ybbB, b0503
COG:	COG2603
UniProt:	P33667
Structures:	\| \|
Complex:
Enzyme type:	selenotransferase/geranyltransferase
Position of modification - modification:	t:34 - mnm5se2U t:34 - mnm5ges2U t:34 - cmnm5ges2U

Comments:

catalyzes conversion: (x5)s2U(x5)se2U. May require SelD? ATP/GTP binding protein. Needs ATP and selenide.

Protein sequence:

MQERHTEQDYRALLIADTPIIDVRAPIEFEHGAMPAAINLPLMNNDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARG
GQRSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQQQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEM
LKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNE
LAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYDPMYRYQLEKKAEKVVFRGEWAEVAEWVKAR

Enzymatic activities:

Reaction	Substrate	Type	Position
cmnm5s2U:cmnm5se2U	tRNA (t)	Gln/UUG/prokaryotic cytosol	34
nm5s2U:nm5se2U	tRNA (t)	Gln/UUG/prokaryotic cytosol	34
mnm5s2U:mnm5se2U	tRNA (t)	Gln/UUG/prokaryotic cytosol	34
cmnm5s2U:cmnm5se2U	tRNA (t)		34
nm5s2U:nm5se2U	tRNA (t)	Lys/SUU/prokaryotic cytosol	34
mnm5s2U:mnm5se2U	tRNA (t)	Lys/SUU/prokaryotic cytosol	34
cmnm5s2U:cmnm5se2U	tRNA (t)		34
nm5s2U:nm5se2U	tRNA (t)	Glu/SUC/prokaryotic cytosol	34
mnm5s2U:mnm5se2U	tRNA (t)	Glu/SUC/prokaryotic cytosol	34
s2U:ges2U	tRNA (t)	Gln/UUG/prokaryotic cytosol	34
cmnm5s2U:cmnm5ges2U	tRNA (t)	Gln/UUG/prokaryotic cytosol	34
mnm5s2U:mnm5ges2U	tRNA (t)	Gln/UUG/prokaryotic cytosol	34
s2U:ges2U	tRNA (t)	Lys/SUU/prokaryotic cytosol	34
mnm5s2U:mnm5ges2U	tRNA (t)	Lys/SUU/prokaryotic cytosol	34
s2U:ges2U	tRNA (t)	Glu/SUC/prokaryotic cytosol	34
mnm5s2U:mnm5ges2U	tRNA (t)	Glu/SUC/prokaryotic cytosol	34

Publications:

Title	Authors	Journal	Details	PubMed Id	DOI
Functional diversity of the rhodanese homology domain: the Escherichia coli ybbB gene encodes a selenophosphate-dependent tRNA 2-selenouridine synthase.	Wolfe MD, Ahmed F, Lacourciere GM, Lauhon CT, Stadtman TC, Larson TJ	J Biol Chem	[details]	14594807	-
Discovery and biological characterization of geranylated RNA in bacteria.	Dumelin CE, Chen Y, Leconte AM, Chen YG, Liu DR...	Nat Chem Biol	[details]	22983156	-

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