Modomics - A Database of RNA Modifications

ID Card:

Full name: TP53 regulating kinase
Synonym: dJ101A2.2 prpk Nori-2p BUD32
GI: 26398348
UniProt: Q96S44
Structures: | 6WQX |
Alpha Fold Predicted Structure: AF-Q96S44-F1


PDB Structures:


6WQX

Structure Description:

Title:
Classification:
Technique:

Abstract of the PDB Structure's related Publication:

Mutations of the p53-related protein kinase (PRPK) and TP53RK-binding protein (TPRKB) cause Galloway-Mowat syndrome (GAMOS) and are found in various human cancers. We have previously shown that small compounds targeting PRPK showed anti-cancer activity against colon and skin cancer. Here we present the 2.53 Å crystal structure of the human PRPK-TPRKB-AMPPNP (adenylyl-imidodiphosphate) complex. The structure reveals details in PRPK-AMPPNP coordination and PRPK-TPRKB interaction. PRPK appears in an active conformation, albeit lacking the conventional kinase activation loop. We constructed a structural model of the human EKC/KEOPS complex, composed of PRPK, TPRKB, OSGEP, LAGE3, and GON7. Disease mutations in PRPK and TPRKB are mapped into the structure, and we show that one mutation, PRPK K238Nfs*2, lost the binding to OSGEP. Our structure also makes the virtual screening possible and paves the way for more rational drug design.

Download RCSB-PDB Structures:

Pdb Files   6WQX.pdb  
Pdbx/mmCIF Files   6WQX.cif  


Protein sequence:

MAAARATTPADGEEPAPEAEALAAARERSSRFLSGLELVKQGAEARVFRGRFQGRAAVIKHRFPKGYRHPALEARLGRRRTVQEARALLRCRRAGISAPVVFFVDYASNCLYMEEIEGSVTVRDYIQSTMETEKTPQGLSNLAKTIGQVLARMHDEDLIHGDLTTSNMLLKPPLEQLNIVLIDFGLSFISALPEDKGVDLYVLEKAFLSTHPNTETVFEAFLKSYSTSSKKARPVLKKLDEVRLRGRKRSMVG

Comments:





Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M A A A R A T T P A D G E E P A P E A E A L A A A R E R S S R F L S G L E L V K Q G A E A R V F R G R F Q G R A A V I K H R F P K G Y R H P A L E A R L G R R R T V Q E A R A L L R C R R A G I S A P V V F F V D Y A S N C L Y M E E I E G S V T V R D Y I Q S T M E T E K T P Q G L S N L A K T I G Q V L A R M H D E D L I H G D L T T S N M L L K P P L E Q L N I V L I D F G L S F I S A L P E D K G V D L Y V L E K A F L S T H P N T E T V F E A F L K S Y S T S S K K A R P V L K K L D E V R L R G R K R S M V G

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q96S44-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q96S44-F1.cif  
DSSP Secondary Structures   Q96S44.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Functional homology between yeast piD261/Bud32 and human PRPK: both phosphorylate p53 and PRPK partially complements piD261/Bud32 deficiency. Facchin S, Lopreiato R, Ruzzene M, Marin O, Sartori G, Gotz C, Montenarh M, Carignani G, Pinna LA... FEBS Lett [details] 12914926 -
Cloning and characterization of a p53-related protein kinase expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes. Abe Y, Matsumoto S, Wei S, Nezu K, Miyoshi A, Kito K, Ueda N, Shigemoto K, Hitsumoto Y, Nikawa J, Enomoto Y... J Biol Chem [details] 11546806 -