Modomics - A Database of RNA Modifications

ID Card:

Full name: Helicase nsp13
Structures: | 6ZSL |
Enzyme type: NTPase, dNTPase, RNA 5′-triphosphatase, RNA helicase, DNA helicase


PDB Structures:


6ZSL

Structure Description:

Title: Crystal structure of the SARS-CoV-2 helicase at 1.94 Angstrom resolution
Classification: HYDROLASE
Technique: X-Ray Diffraction
Resolution: 1.94
R value free: 0.253
R value observed: 0.211
R value work: 0.209

Abstract of the PDB Structure's related Publication:

There is currently a lack of effective drugs to treat people infected with SARS-CoV-2, the cause of the global COVID-19 pandemic. The SARS-CoV-2 Non-structural protein 13 (NSP13) has been identified as a target for anti-virals due to its high sequence conservation and essential role in viral replication. Structural analysis reveals two "druggable" pockets on NSP13 that are among the most conserved sites in the entire SARS-CoV-2 proteome. Here we present crystal structures of SARS-CoV-2 NSP13 solved in the APO form and in the presence of both phosphate and a non-hydrolysable ATP analog. Comparisons of these structures reveal details of conformational changes that provide insights into the helicase mechanism and possible modes of inhibition. To identify starting points for drug development we have performed a crystallographic fragment screen against NSP13. The screen reveals 65 fragment hits across 52 datasets opening the way to structure guided development of novel antiviral agents.

Download RCSB-PDB Structures:

Pdb Files   6ZSL.pdb  
Pdbx/mmCIF Files   6ZSL.cif  


Protein sequence:

AVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCNAPGCDVTDVTQLYLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDFNAIATCDWTNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDRELHLSWEVGKPRPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVGDYFVLTSHTVMPLSAPTLVPQEHYVRITGLYPTLNISDEFSSNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARARVECFDKFKVNSTLEQYVFCTVNALPETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQLPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLKAHKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFISPYNSQNAVASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNVAITRAKIGILCIMSDRDLYDKLQFTSLEIPRRNVATLQ

Comments:

Multi-functional protein with NTPase, dNTPase, RNA 5′-triphosphatase, RNA helicase, and DNA helicase activities. Nsp13 function directly upstream of the GTase in the m7G capping pathway by acting as a 5’ triphosphatase.







Publications: