Modomics - A Database of RNA Modifications

ID Card:

Full name: Pseudouridine synthase
GI: 74751577
UniProt: Q8WWH5
Structures: | |
Alpha Fold Predicted Structure: AF-Q8WWH5-F1
Enzyme type: pseudouridine synthase



Protein sequence:

MAASEAAVVSSPSLKTDTSPVLETAGTVAAMAATPSARAAAAVVAAAARTGSEARVSKAALATKLLSLSGVFAVHKPKGPTSAELLNRLKEKLLAEAGMPSPEWTKRKKQTLKIGHGGTLDSAARGVLVVGIGSGTKMLTSMLSGSKRYTAIGELGKATDTLDSTGRVTEEKPYDKITQEDIEGILQKFTGNIMQVPPLYSALKKDGQRLSTLMKRGEVVEAKPARPVTVYSISLQKFQPPFFTLDVECGGGFYIRSLVSDIGKELSSCANVLELTRTKQGPFTLEEHALPEDKWTIDDIAQSLEHCSSLFPAELALKKSKPESNEQVLSCEYITLNEPKREDDVIKTC

Comments:

TRUB1-dependent mRNA/tRNA pseudouridylation occurs in the nucleus. Characterization of TRUB1 targets demonstrates the requirement for a well-defined stem and loop structure for achieving pseudouridylation.





Alpha Fold Predicted Structure:






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Protein sequence:

M A A S E A A V V S S P S L K T D T S P V L E T A G T V A A M A A T P S A R A A A A V V A A A A R T G S E A R V S K A A L A T K L L S L S G V F A V H K P K G P T S A E L L N R L K E K L L A E A G M P S P E W T K R K K Q T L K I G H G G T L D S A A R G V L V V G I G S G T K M L T S M L S G S K R Y T A I G E L G K A T D T L D S T G R V T E E K P Y D K I T Q E D I E G I L Q K F T G N I M Q V P P L Y S A L K K D G Q R L S T L M K R G E V V E A K P A R P V T V Y S I S L Q K F Q P P F F T L D V E C G G G F Y I R S L V S D I G K E L S S C A N V L E L T R T K Q G P F T L E E H A L P E D K W T I D D I A Q S L E H C S S L F P A E L A L K K S K P E S N E Q V L S C E Y I T L N E P K R E D D V I K T C

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q8WWH5-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q8WWH5-F1.cif  
DSSP Secondary Structures   Q8WWH5.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
A complete landscape of post-transcriptional modifications in mammalian mitochondrial tRNAs. Suzuki T, Suzuki T... Nucleic Acids Res [details] 24831542 -
Mammalian nuclear TRUB1, mitochondrial TRUB2, and cytoplasmic PUS10 produce conserved pseudouridine 55 in different sets of tRNA. Shaoni Mukhopadhyay,Manisha Deogharia,Ramesh Gupta RNA [details] 33023933 -
TRUB1 is the predominant pseudouridine synthase acting on mammalian mRNA via a predictable and conserved code. Modi Safra,Ronit Nir,Daneyal Farouq,Ilya Vainberg Slutskin,Schraga Schwartz Genome Res [details] 28073919 -