Modomics - A Database of RNA Modifications

ID Card:

Full name: " tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial"
UniProt: Q8CCT7
Alpha Fold Predicted Structure: AF-Q8CCT7-F1
Enzyme type: methyltransferase



Protein sequence:

MLTRLKAKSEGKLAKQLCRVVLDQFDKQYSKELGDSWSTVRDVLISPSLWQYAILFNRFNYPFELEKALHLRGYHTVLQGALPHYPKSMKCYLSRTPDRMPSERHQTGSLKKYYLLNAASLLPVLALELRDGEAVLDLCAAPGGKSVALLQCAYPGYLLCNEYDRPRGRWLRQTLESFIPQPLINVIKVSELDGREMGDAQPATFDKVLVDAPCSNDRSWLFSSDSQKAAYRIHQRKNLPVLQVELVRSAIKALRPGGLLVYSTCTLSKAENQDVISEVLTSDSNIVPVDISGIARTFSQDFTFAPTDQKCSLLVIPEKGKAWGPMYIAKLKKGMSTRKRQGEFCKPC

Comments:

A mitochondrial tRNA modification enzyme, NSUN3, is found to be essential for embryonic development in mice, with whole-body knockout causing embryonic lethality and heart-specific Nsun3 knockout leading to heart aberration in older mice. Human NSUN3 mutations are associated with mitochondrial diseases. Mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C) at position 34 of mt-tRNA(Met). mt-tRNA(Met) methylation at cytosine34 takes place at the wobble position of the anticodon and initiates the formation of 5-formylcytosine (f5c) at this position. mt-tRNA(Met) containing the f5c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation.





Alpha Fold Predicted Structure:






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Protein sequence:

M L T R L K A K S E G K L A K Q L C R V V L D Q F D K Q Y S K E L G D S W S T V R D V L I S P S L W Q Y A I L F N R F N Y P F E L E K A L H L R G Y H T V L Q G A L P H Y P K S M K C Y L S R T P D R M P S E R H Q T G S L K K Y Y L L N A A S L L P V L A L E L R D G E A V L D L C A A P G G K S V A L L Q C A Y P G Y L L C N E Y D R P R G R W L R Q T L E S F I P Q P L I N V I K V S E L D G R E M G D A Q P A T F D K V L V D A P C S N D R S W L F S S D S Q K A A Y R I H Q R K N L P V L Q V E L V R S A I K A L R P G G L L V Y S T C T L S K A E N Q D V I S E V L T S D S N I V P V D I S G I A R T F S Q D F T F A P T D Q K C S L L V I P E K G K A W G P M Y I A K L K K G M S T R K R Q G E F C K P C
50100150200250300SequenceGHTBSN

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q8CCT7-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q8CCT7-F1.cif  
DSSP Secondary Structures   Q8CCT7.dssp  





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