Modomics - A Database of RNA Modifications

ID Card:

Full name: E3 ubiquitin-protein ligase HAKAI homolog
UniProt: Q9LFC0
Alpha Fold Predicted Structure: AF-Q9LFC0-F1
Enzyme type: ligase



Protein sequence:

MLQIRLRRDSPTETGNGARPSPTETVTVACPDHLVLADLPVAKGIGSVTPTTVIKPVGRRSRRQLGERVHFCVRCDFPIAIYGRLIPCDHAFCLECARSDSICYLCDERIQKIQTIKMMEGIFICAAPHCLRSFLKKLDFEAHVHDLHGSLLQADAEKEDGNQSDVQSTMQQSSASESTLRAPLRSQLQQSRELNRSASFAKSQSGFSQVQNYPPDSDNSRPPGFETASPKPGIRFPDYPQPMNLMQPPSLPVPMNQNPGLPQQFGFPSYPTTESGSSQQFFNGAQYEMTRTESGGSEQSSLLGYPPPSPMTNLNFQGSYPPPSWNPGMAPPHTTQQVNRGRDGQSFGWPQENRDGFGQE

Comments:

"Probable E3 ubiquitin-protein ligase which is a subunit of the N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation at the 5'-[AG]GAC-3' consensus sites of some mRNAs. Associates with MTA, MTB, FIP37 and VIR to form the m6A writer complex which is essential for adenosine methylation at specific mRNA sequences. N6-methyladenosine (m6A) plays a role in mRNA stability, processing, translation efficiency and editing."





Alpha Fold Predicted Structure:






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Protein sequence:

M L Q I R L R R D S P T E T G N G A R P S P T E T V T V A C P D H L V L A D L P V A K G I G S V T P T T V I K P V G R R S R R Q L G E R V H F C V R C D F P I A I Y G R L I P C D H A F C L E C A R S D S I C Y L C D E R I Q K I Q T I K M M E G I F I C A A P H C L R S F L K K L D F E A H V H D L H G S L L Q A D A E K E D G N Q S D V Q S T M Q Q S S A S E S T L R A P L R S Q L Q Q S R E L N R S A S F A K S Q S G F S Q V Q N Y P P D S D N S R P P G F E T A S P K P G I R F P D Y P Q P M N L M Q P P S L P V P M N Q N P G L P Q Q F G F P S Y P T T E S G S S Q Q F F N G A Q Y E M T R T E S G G S E Q S S L L G Y P P P S P M T N L N F Q G S Y P P P S W N P G M A P P H T T Q Q V N R G R D G Q S F G W P Q E N R D G F G Q E

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-Q9LFC0-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-Q9LFC0-F1.cif  
DSSP Secondary Structures   Q9LFC0.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
RNA methyltransferases in plants: Breakthroughs in function and evolution Ricardo Ferraz 1, Sílvia Coimbra 2, Sandra Correia 3, Jorge Canhoto 4 [details] 36502609 10.1016/j.plaphy.2022.12.001
Identification of factors required for m6 A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI Kamil Růžička 1 2, Mi Zhang 3, Ana Campilho 2 4, Zsuzsanna Bodi 3, Muhammad Kashif 2, Mária Saleh 1, Dominique Eeckhout 5 6, Sedeer El-Showk 2, Hongying Li 3 7, Silin Zhong 3 8, Geert De Jaeger 5 6, Nigel P Mongan 9, Jan Hejátko 1, Ykä Helariutta 2 10, Rupert G Fray [details] 28503769 10.1111/nph.14586