Modomics - A Database of RNA Modifications

ID Card:

Full name: Mitochondrial tRNA-specific 2-thiouridylase 1
Synonym: MTU1, TRMT1
GI: 31542641
COG: COG0482
UniProt: O75648
Alpha Fold Predicted Structure: AF-O75648-F1
Enzyme type: sulfurtransferase
Position of modification - modification: t:34 - tm5s2U



Protein sequence:

MQALRHVVCALSGGVDSAVAALLLRRRGYQVTGVFMKNWDSLDEHGVCTADKDCEDAYRVCQILDIPFHQVSYVKEYWNDVFSDFLNEYEKGRTPNPDIVCNKHIKFSCFFHYAVDNLGADAIATGHYARTSLEDEEVFEQKHVKKPEGLFRNRFEVRNAVKLLQAADSFKDQTFFLSQVSQDALRRTIFPLGGLTKEFVKKIAAENRLHHVLQKKESMGMCFIGKRNFEHFLLQYLQPRPGHFISIEDNKVLGTHKGWFLYTLGQRANIGGLREPWYVVEKDSVKGDVFVAPRTDHPALYRDLLRTSRVHWIAEEPPAALVRDKMMECHFRFRHQMALVPCVLTLNQDGTVWVTAVQAVRALATGQFAVFYKGDECLGSGKILRLGPSAYTLQKGQRRAGMATESPSDSPEDGPGLSPLL

Comments:

Homolog of yeast mitochondrial ATP-dependent tRNA sulfurtransferase Mtu1. Sulfur group originates from cysteine via a cascade of sulphur relay proteins, including the pyridoxal phosphate-containing cysteine desulfurase Nfs1 and at least a third mitochondrial sulfur transport protein. In mitochondria of T. brucei (Eukarya), this third protein partner is Isd11.




Reaction Substrate SubstrateType Position (Anti)Codon Modified (Anti)Codon Amino Acid Change Transcript Name Transcript Region Cellular Localization References
U:s2U RNA tRNA 34 UUU 2UU tRNALysUUU wobble - position Mitochondrion 15509579   
cmnm5U:cmnm5s2U RNA tRNA 34 !UU $UU tRNALys!UU wobble - position Mitochondrion 15509579   
nm5U:nm5s2U RNA tRNA 34 ∪UU ∫UU tRNALys∪UU wobble - position Mitochondrion 15509579   
mnm5U:mnm5s2U RNA tRNA 34 {UU SUU tRNALys{UU wobble - position Mitochondrion 15509579   
mcm5U:mcm5s2U RNA tRNA 34 {UU 3UU tRNALys{UU wobble - position Mitochondrion 15509579   
tm5U:tm5s2U RNA tRNA 34 ÊUU ∃UU tRNALysGUA wobble - position Mitochondrion 15509579   
m5U:m5s2U RNA tRNA 34 TUU 3UU tRNALysTUU wobble - position Mitochondrion 15509579   
Um:s2Um RNA tRNA 34 JUU ∏UU tRNALysJUU wobble - position Mitochondrion

Alpha Fold Predicted Structure:






Clear Selection and Reset Camera

Protein sequence:

M Q A L R H V V C A L S G G V D S A V A A L L L R R R G Y Q V T G V F M K N W D S L D E H G V C T A D K D C E D A Y R V C Q I L D I P F H Q V S Y V K E Y W N D V F S D F L N E Y E K G R T P N P D I V C N K H I K F S C F F H Y A V D N L G A D A I A T G H Y A R T S L E D E E V F E Q K H V K K P E G L F R N R F E V R N A V K L L Q A A D S F K D Q T F F L S Q V S Q D A L R R T I F P L G G L T K E F V K K I A A E N R L H H V L Q K K E S M G M C F I G K R N F E H F L L Q Y L Q P R P G H F I S I E D N K V L G T H K G W F L Y T L G Q R A N I G G L R E P W Y V V E K D S V K G D V F V A P R T D H P A L Y R D L L R T S R V H W I A E E P P A A L V R D K M M E C H F R F R H Q M A L V P C V L T L N Q D G T V W V T A V Q A V R A L A T G Q F A V F Y K G D E C L G S G K I L R L G P S A Y T L Q K G Q R R A G M A T E S P S D S P E D G P G L S P L L

Secondary Structure Alphabet

  • G: 3-turn helix (310helix)
  • H: α-helix
  • I: 𝝅-helix (5 - turn helix)
  • T: Hydrogen Bonded Turn
  • B: β-sheet
  • S: Bend
  • C: Coil (residues not present in any of the above conformations)
  • N: Not assigned

Download PDB Structures & DSSP Secondary Structures:

Alpha Fold Pdb Files   AF-O75648-F1.pdb  
Alpha Fold Pdbx/mmCIF Files   AF-O75648-F1.cif  
DSSP Secondary Structures   O75648.dssp  





Publications:

Title Authors Journal Details PubMed Id DOI
Human TRMU encoding the mitochondrial 5-methylaminomethyl-2-thiouridylate-methyltransferase is a putative nuclear modifier gene for the phenotypic expression of the deafness-associated 12S rRNA mutations. Yan Q, Bykhovskaya Y, Li R, Mengesha E, Shohat M, Estivill X, Fischel-Ghodsian N, Guan MX Biochem Biophys Res Commun [details] 16513084 -
Mitochondria-specific RNA-modifying enzymes responsible for the biosynthesis of the wobble base in mitochondrial tRNAs. Implications for the molecular pathogenesis of human mitochondrial diseases. Umeda N, Suzuki T, Yukawa M, Ohya Y, Shindo H, Watanabe K, Suzuki T J Biol Chem [details] 15509579 -
Human mitochondrial tRNAs: biogenesis, function, structural aspects, and diseases. Suzuki T, Nagao A, Suzuki T Annu Rev Genet [details] 21910628 -
Human mitochondrial diseases caused by lack of taurine modification in mitochondrial tRNAs. Suzuki T, Nagao A, Suzuki T Wiley Interdiscip Rev RNA [details] 21957023 -
The 2-thiouridylase function of the human MTU1 (TRMU) enzyme is dispensable for mitochondrial translation. Sasarman F, Antonicka H, Horvath R, Shoubridge EA Hum Mol Genet [details] 21890497 -

Links:

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