ID Card:
| Full name: |
16S rRNA (adenine(1408)-N(1))-methyltransferase NpmC |
| Enzyme type: |
|
| Position of modification - modification: |
:1408(None) - m1A
|
| Level of experimental evidence: |
3 |
| Level of experimental reliability: |
3 |
Protein sequence:
Comments:
NpmC, was identified after analysisng all world-wide available metagenomic projects in a One Health context. This gene has a high level of similarity (91.5%) with npmA and up to 92.7% similarity at amino acidic level. The protein encoded by this gene presents the conserved motifs required for A1408 methylation. npmC was synthesized and its expression in Escherichia coli resulted in a high level of resistance to 4,5-disubstituted 2-deoxystreptamine (2-DOS) and 4-monosubstituted 2-DOS aminoglycosides, as well as moderate resistance to 4,6-disusbstituted 2-DOS aminoglycosides, including the last resort aminoglycoside, plazomicin.
| Reaction |
Substrate |
SubstrateType |
Position |
(Anti)Codon |
Modified (Anti)Codon |
Amino Acid Change |
Transcript Name |
Transcript Region |
Cellular Localization |
References |
|
A:m1A
|
rRNA (r) |
rRNA/rRNA/prokaryotic cytosol |
1408 |
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Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| NpmC - a novel A1408 16S rRNA methyltransferase in the gut of humans and animals. |
Matamoros BR; Serna C; Wedel E; Montero N; Kirpekar F; Gonzalez-Zorn B |
Int J Antimicrob Agents |
[details]
|
39522830
|
10.1016/j.ijantimicag.2024.107382
|