ID Card:
| Full name: |
tRNA(Thr) (cytosine(32)-N(3))-methyltransferase |
| Enzyme type: |
|
| Position of modification - modification: |
t:None - m3C
|
| Level of experimental evidence: |
2 |
| Level of experimental reliability: |
2 |
Protein sequence:
Comments:
Trm140 in Trypanosoma brucei plays a crucial and unique role in tRNA modification at position 32, specifically catalyzing the formation of m3C in the anticodon loop of several tRNAs including tRNA Thr. What distinguishes TbTrm140 is its strict functional interdependence and synergy with the ADAT2/3 deaminase complex, which catalyzes subsequent deamination to form m3U at the same position.
| Reaction |
Substrate |
SubstrateType |
Position |
(Anti)Codon |
Modified (Anti)Codon |
Amino Acid Change |
Transcript Name |
Transcript Region |
Cellular Localization |
References |
|
C:m3C
|
tRNA (t) |
tRNA/tRNA/eukaryotic cytosol |
32 |
|
|
|
|
|
|
|
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| Editing and methylation at a single site by functionally interdependent activities. |
Rubio MA; Gaston KW; McKenney KM; Fleming IM; Paris Z; Limbach PA; Alfonzo JD |
Nature |
[details]
|
28230119
|
10.1038/nature21396
|