ID Card:
| Full name: |
U6 snRNA (guanine-N(2))-methyltransferase |
| UniProt: |
Q9BTF0 |
| Complex: |
THUMPD2 -TRM112 |
| Enzyme type: |
|
| Position of modification - modification: |
n:72 - m2G
|
| Level of experimental evidence: |
2 |
| Level of experimental reliability: |
2 |
Protein sequence:
MSEARGEPGSGPEAGARFFCTAGRGLEPFVMREVRARLAATQVEYISGKVFFTTCSDLNMLKKLKSAERLFLLIKKQFPLIISSVSKGKIFNEMQRLINEDPGSWLNAISIWKNLLELDAKKEKLSQRDDNQLKRKVGENEIIAKKLKIEQMQKIEENRDCQLEKQIKEETLEQRDFTTKSEKFQEEEFQNDIEKAIDTHNQNDLTFRVSCRCSGTIGKAFTAQEVGKVIGIAIMKHFGWKADLRNPQLEIFIHLNDIYSVVGIPVFRVSLASRAYIKTAGLRSTIAWAMASLADIKAGAFVLDPMCGLGTILLEAAKEWPDVYYVGADVSDSQLLGTWDNLKAAGLEDKIELLKISVIELPLPSESVDIIISDIPFGKKFKLGKDIKSILQEMERVLHVGGTIVLLLSEDHHRRLTDCKESNIPFNSKDSHTDEPGIKKCLNPEEKTGAFKTASTSFEASNHKFLDRMSPFGSLVPVECYKVSLGKTDAFICKYKKSHSSGL
Comments:
Catalytic subunit of the THUMPD2-TRM112 methyltransferase complex, that specifically mediates the S-adenosyl-L-methionine-dependent N2-methylation of guanosine nucleotides most probably at position 72 (m2G72), in the U6snRNA of the major spliceosome.
| Reaction |
Substrate |
SubstrateType |
Position |
(Anti)Codon |
Modified (Anti)Codon |
Amino Acid Change |
Transcript Name |
Transcript Region |
Cellular Localization |
References |
|
G:m2G
|
snRNA (sn) |
snRNA/snRNA/eukaryotic nucleoplasm |
72 |
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Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| THUMPD2 catalyzes the N2-methylation of U6 snRNA of the spliceosome catalytic center and regulates pre-mRNA splicing and retinal degeneration. |
Yang WQ; Ge JY; Zhang X; Zhu WY; Lin L; Shi Y; Xu B; Liu RJ |
Nucleic Acids Res |
[details]
|
38165050
|
10.1093/nar/gkad1243
|
| N 2-methylguanosine modifications on human tRNAs and snRNA U6 are important for cell proliferation, protein translation and pre-mRNA splicing. |
Wang C; Ulryck N; Herzel L; Pythoud N; Kleiber N; Guérineau V; Jactel V; Moritz C; Bohnsack MT; Carapito C; Touboul D; Bohnsack KE; Graille M |
Nucleic Acids Res |
[details]
|
37283053
|
10.1093/nar/gkad487
|