| Full name: | tRNA (guanine(6)-N(2))-methyltransferase |
|---|---|
| UniProt: | Q9BV44 |
| Complex: | THUMPD3 - TRM112 |
| Enzyme type: | |
| Position of modification - modification: |
t:None - m2G t:None - m2G |
| Level of experimental evidence: | 2 |
| Level of experimental reliability: | 2 |
MCDIEEATNQLLDVNLHENQKSVQVTESDLGSESELLVTIGATVPTGFEQTAADEVREKLGSSCKISRDRGKIYFVISVESLAQVHCLRSVDNLFVVVQEFQDYQFKQTKEEVLKDFEDLAGKLPWSNPLKVWKINASFKKKKAKRKKINQNSSKEKINNGQEVKIDQRNVKKEFTSHALDSHILDYYENPAIKEDVSTLIGDDLASCKDETDESSKEETEPQVLKFRVTCNRAGEKHCFTSNEAARDFGGAVQDYFKWKADMTNFDVEVLLNIHDNEVIVGIALTEESLHRRNITHFGPTTLRSTLAYGMLRLCDPLPYDIIVDPMCGTGAIPIEGATEWSDCFHIAGDNNPLAVNRAANNIASLLTKSQIKEGKPSWGLPIDAVQWDICNLPLRTGSVDIIVTDLPFGKRMGSKKRNWNLYPACLREMSRVCTPTTGRAVLLTQDTKCFTKALSGMRHVWRKVDTVWVNVGGLRAAVYVLIRTPQAFVHPSEQDGERGTLWQCKE
Catalytic subunit of the THUMPD3-TRM112 methyltransferase complex, that specifically mediates the S-adenosyl-L-methionine-dependent N2-methylation of guanosine nucleotide at position 6 (m2G) in tRNAs. Also catalyzes the S-adenosyl-L-methionine-dependent N2-methylation of guanosine nucleotide at position 7 of tRNA(Trp).
| Reaction | Substrate | SubstrateType | Position | (Anti)Codon | Modified (Anti)Codon | Amino Acid Change | Transcript Name | Transcript Region | Cellular Localization | References |
|---|---|---|---|---|---|---|---|---|---|---|
| G:m2G | tRNA (t) | tRNA/tRNA/eukaryotic cytosol | 6 | |||||||
| G:m2G | tRNA (t) | tRNA/tRNA/eukaryotic cytosol | 7 |
| Title | Authors | Journal | Details | ||
|---|---|---|---|---|---|
| THUMPD3-TRMT112 is a m2G methyltransferase working on a broad range of tRNA substrates. | Yang W.Q., Xiong Q.P., Ge J.Y., Li H., Zhu W.Y., Nie Y., Lin X., Lv D., Li J., Lin H., Liu R.J. | Nucleic Acids Res | [details] | 34669960 | 10.1093/nar/gkab927 |
| N 2-methylguanosine modifications on human tRNAs and snRNA U6 are important for cell proliferation, protein translation and pre-mRNA splicing. | Wang C; Ulryck N; Herzel L; Pythoud N; Kleiber N; Guérineau V; Jactel V; Moritz C; Bohnsack MT; Carapito C; Touboul D; Bohnsack KE; Graille M | Nucleic Acids Res | [details] | 37283053 | 10.1093/nar/gkad487 |