| Full name: | 5-cytosine rRNA methyltransferase NSUN4 |
|---|---|
| UniProt: | Q96CB9 |
| Complex: | MTERF4 |
| Enzyme type: | |
| Position of modification - modification: |
:911(None) - m5C |
| Level of experimental evidence: | 2 |
| Level of experimental reliability: | 2 |
MAALTLRGVRELLKRVDLATVPRRHRYKKKWAATEPKFPAVRLALQNFDMTYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDHVSAKLEQLSAKDFVNEAISHWELQSEGGQSAAPSPASWACSPNLRCFTFDRGDISRFPPARPGSLGVMEYYLMDAASLLPVLALGLQPGDIVLDLCAAPGGKTLALLQTGCCRNLAANDLSPSRIARLQKILHSYVPEEIRDGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFKRSRKKERQILPVLQVQLLAAGLLATKPGGHVVYSTCSLSHLQNEYVVQGAIELLANQYSIQVQVEDLTHFRRVFMDTFCFFSSCQVGELVIPNLMANFGPMYFCKMRRLT
NSUN4 methylates cytosine 911 in 12S rRNA (m5C911) of the SSU. NSUN4, which forms a complex with MTERF4. NSUN4 does not need MTERF4 to generate the modification. Instead, the NSUN4/MTERF4 complex is required to assemble the SSU and LSU to form a monosome. NSUN4 is thus a dual function protein, which on the one hand is needed for 12S rRNA methylation and, on the other hand interacts with MTERF4 to facilitate monosome assembly.
| Reaction | Substrate | SubstrateType | Position | (Anti)Codon | Modified (Anti)Codon | Amino Acid Change | Transcript Name | Transcript Region | Cellular Localization | References |
|---|---|---|---|---|---|---|---|---|---|---|
| C:m5C | rRNA (r) | rRNA/rRNA/eukaryotic mitochondria | 911 |
| Title | Authors | Journal | Details | ||
|---|---|---|---|---|---|
| NSUN4 is a dual function mitochondrial protein required for both methylation of 12S rRNA and coordination of mitoribosomal assembly. | Metodiev MD, Spåhr H, Loguercio Polosa P, Meharg C, Becker C, Altmueller J, Habermann B, Larsson NG, Ruzzenente B. | PLoS Genet. | [details] | 24516400 | 10.1371/journal.pgen.1004110 |