ID Card:
Full name:
Trimethylguanosine synthase 1
Synonym:
P2573
GI:
74583797
Orf:
YPL157W
COG:
COG0116
UniProt:
Q12052
Alpha Fold Predicted Structure:
AF-Q12052-F1
Enzyme type:
methyltransferase
Position of modification - modification:
n :0 - m2,2,7GpppN
Protein sequence:
MGRTFIHASKIKHAARKRKHHSNFRTLIKLLNNDAYKIESSKPLKNGKLFKYWKNRRRLFSKIDSASIYMTDELWFSVTPERIACFLANFVKACMPNAERILDVFCGGGGNTIQFAMQFPYVYGVDYSIEHIYCTAKNAQSYGVDDRIWLKRGSWKKLVSKQKLSKIKYDCVFGSPPWGGPEYLRNDVYDLEQHLKPMGITKMLKSFLKLSPNVIMFLPRNSDLNQLSRATRKVLGPFAKCKVLYVKENGYMKGIFCMWGECFFNYEPASTENSRRESSEKEELSSENEELSKRKKHESTTTTKDNTVDIYDVNG
Comments:
Tgs1p is an evolutionarily conserved protein that carries a signature of S-AdoMet methyltransferase and is essential for hypermethylation of the m(7)G caps of both snRNAs and snoRNAs (Mouaikel et al. 2002 ). The absence of Tgs1 caused changes in telomere length and structure, improved telomeric silencing and stabilized telomeric recombination. Moreover, the yeast snRNA and snoRNA methyltransferase Tgs1 is responsible for TLC1 m3G cap formation (Franke et al. 2008 ).
Reaction
Substrate
SubstrateType
Position
(Anti)Codon
Modified (Anti)Codon
Amino Acid Change
Transcript Name
Transcript Region
Cellular Localization
References
m7G:m2,7G
RNA
snoRNA
1
N - terminus
Nucleolus
11983179
m2,7G:m2,2,7G
RNA
snoRNA
1
N - terminus
Nucleolus
11983179
m7G:m2,7G
RNA
snRNA
1
U1, U2, U4 and U5
N - terminus
Nucleus
11983179
m2,7G:m2,2,7G
RNA
snRNA
1
U1, U2, U4 and U5
N - terminus
Nucleus
11983179
Alpha Fold Predicted Structure:
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Protein sequence:
M
G
R
T
F
I
H
A
S
K
I
K
H
A
A
R
K
R
K
H
H
S
N
F
R
T
L
I
K
L
L
N
N
D
A
Y
K
I
E
S
S
K
P
L
K
N
G
K
L
F
K
Y
W
K
N
R
R
R
L
F
S
K
I
D
S
A
S
I
Y
M
T
D
E
L
W
F
S
V
T
P
E
R
I
A
C
F
L
A
N
F
V
K
A
C
M
P
N
A
E
R
I
L
D
V
F
C
G
G
G
G
N
T
I
Q
F
A
M
Q
F
P
Y
V
Y
G
V
D
Y
S
I
E
H
I
Y
C
T
A
K
N
A
Q
S
Y
G
V
D
D
R
I
W
L
K
R
G
S
W
K
K
L
V
S
K
Q
K
L
S
K
I
K
Y
D
C
V
F
G
S
P
P
W
G
G
P
E
Y
L
R
N
D
V
Y
D
L
E
Q
H
L
K
P
M
G
I
T
K
M
L
K
S
F
L
K
L
S
P
N
V
I
M
F
L
P
R
N
S
D
L
N
Q
L
S
R
A
T
R
K
V
L
G
P
F
A
K
C
K
V
L
Y
V
K
E
N
G
Y
M
K
G
I
F
C
M
W
G
E
C
F
F
N
Y
E
P
A
S
T
E
N
S
R
R
E
S
S
E
K
E
E
L
S
S
E
N
E
E
L
S
K
R
K
K
H
E
S
T
T
T
T
K
D
N
T
V
D
I
Y
D
V
N
G
Secondary Structure Alphabet
G: 3-turn helix (310 helix) H: α-helix I: 𝝅-helix (5 - turn helix) T: Hydrogen Bonded Turn B: β-sheet S: Bend C: Coil (residues not present in any of the above conformations) N: Not assigned
Download PDB Structures & DSSP Secondary Structures:
Publications:
Title
Authors
Journal
Details
PubMed Id DOI
Specificity and mechanism of RNA cap guanine-N2 methyltransferase (Tgs1).
Hausmann S, Shuman S
J Biol Chem
[details]
15590684
-
Hypermethylation of the cap structure of both yeast snRNAs and snoRNAs requires a conserved methyltransferase that is localized to the nucleolus.
Mouaikel J, Verheggen C, Bertrand E, Tazi J, Bordonne R
Mol Cell
[details]
11983179
-
Sequence-structure-function relationships of Tgs1, the yeast snRNA/snoRNA cap hypermethylase.
Mouaikel J, Bujnicki JM, Tazi J, Bordonne R
Nucleic Acids Res
[details]
12907733
-
Hypermethylation of yeast telomerase RNA by the snRNA and snoRNA methyltransferase Tgs1.
Franke J, Gehlen J, Ehrenhofer-Murray AE
J Cell Sci
[details]
18840651
-