ID Card:
| Full name: |
tRNA 2-thiolation protein |
| Synonym: |
Tuc1, HVO_0580 |
| UniProt: |
D4GSH6 |
| Enzyme type: |
|
| Position of modification - modification: |
t:None - mcm5s2U
|
| Level of experimental evidence: |
2 |
| Level of experimental reliability: |
2 |
Protein sequence:
MECDKCGRDAVMHAAYSGAHLCDDHFCASVEKRVRRRIREDNMLPRDASPENPQTWVIGLSGGKDSVVLTHILDDTFGRDPRIELVALTIHEGIEGYRDKSVDACVELAEDLDIHHELVTYEDEFGVQMDDVVEKDPENMAACAYCGVFRRDLLERFADELGADKLLTGHNLDDEAQTALMNFFEGDLKQVAKHFDASIGDFEKRRDAGEFIPRAKPLRDVPEKEVALYAHLKDLPAHITECPHSSEAYRGEIQQLLLKLEENHPGTRHSIMAGYEELAELTAREYRGEGRVDLNDCERCGSKTAGDVCRKCRLIESIEAV
Comments:
Required for thiolation of mcm5S2U at the wobble uridine position of tRNA specific for lysine (tRNA(Lys)). Probably acts by catalyzing adenylation of tRNA, an intermediate required for 2-thiolation. May also act as a sulfurtransferase that transfers sulfur from thiocarboxylated SAMP2 onto the uridine of tRNA at wobble position. Required for cell growth at elevated temperatures.
| Reaction |
Substrate |
SubstrateType |
Position |
(Anti)Codon |
Modified (Anti)Codon |
Amino Acid Change |
Transcript Name |
Transcript Region |
Cellular Localization |
References |
|
mcm5U:mcm5s2U
|
tRNA (t) |
tRNA/tRNA/prokaryotic cytosol |
34 |
|
|
|
|
|
|
|
Publications:
| Title |
Authors |
Journal |
Details |
PubMed Id |
DOI |
| RNomics and Modomics in the halophilic archaea Haloferax volcanii: identification of RNA modification genes. |
Grosjean H; Gaspin C; Marck C; Decatur WA; de Crécy-Lagard V |
BMC Genomics |
[details]
|
18844986
|
10.1186/1471-2164-9-470
|
| Archaeal Tuc1/Ncs6 homolog required for wobble uridine tRNA thiolation is associated with ubiquitin-proteasome, translation, and RNA processing system homologs. |
Chavarria NE; Hwang S; Cao S; Fu X; Holman M; Elbanna D; Rodriguez S; Arrington D; Englert M; Uthandi S; Söll D; Maupin-Furlow JA |
PLoS One |
[details]
|
24906001
|
10.1371/journal.pone.0099104
|